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Literature summary extracted from

  • Wirtz, M.; Hell, R.
    Production of cysteine for bacterial and plant biotechnology: Application of cysteine feedback-insensitive isoforms of serine acetyltransferase (2003), Amino Acids, 24, 195-203.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.30 expression in inactivated Escherichia coli mutant, insensitive against inhibition by L-cysteine Nicotiana tabacum
2.3.1.30 expression in inactivated Escherichia coli mutant, unaltered L-cysteine sensitivity compared to wild-type Nicotiana tabacum
2.3.1.30 insertional inactivation of wild-type CysE gene from Escherichia coli C600 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.30 additional information
-
 Escherichia coli
2.3.1.30 additional information transformation via Agrobacterium tumefaciens, transgenic Nicotiana tabacum plants overexpressing recombinant Arabidopsis thaliana SAT A isozyme, expression with and without mitochondrial transit peptide Nicotiana tabacum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.30 acetyl-CoA + L-serine Escherichia coli
-
 CoA + O-acetyl-L-serine
-
 ?
2.3.1.30 acetyl-CoA + L-serine Nicotiana tabacum
-
 CoA + O-acetyl-L-serine
-
 ?
2.3.1.30 acetyl-CoA + L-serine Arabidopsis thaliana
-
 CoA + O-acetyl-L-serine
-
 ?
2.3.1.30 acetyl-CoA + L-serine Escherichia coli C600
-
 CoA + O-acetyl-L-serine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.30 Arabidopsis thaliana Q39218
-
-
2.3.1.30 Arabidopsis thaliana Q42538
-
-
2.3.1.30 Arabidopsis thaliana Q42588
-
-
2.3.1.30 Escherichia coli
-
 CysE gene
-
2.3.1.30 Escherichia coli C600
-
 CysE gene
-
2.3.1.30 Nicotiana tabacum
-
 cv. SNN
-
2.3.1.30 Nicotiana tabacum Q8H0P5 gene SAT7
-
2.3.1.30 Nicotiana tabacum Q8H0P6 gene SAT4
-
2.3.1.30 Nicotiana tabacum Q8H0P7 gene SAT1
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.30 0.0025
-
 wild-type strain C600 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.30 acetyl-CoA + L-serine
-
 Escherichia coli CoA + O-acetyl-L-serine
-
 ?
2.3.1.30 acetyl-CoA + L-serine
-
 Nicotiana tabacum CoA + O-acetyl-L-serine
-
 ?
2.3.1.30 acetyl-CoA + L-serine
-
 Arabidopsis thaliana CoA + O-acetyl-L-serine
-
 ?
2.3.1.30 acetyl-CoA + L-serine
-
 Escherichia coli C600 CoA + O-acetyl-L-serine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.30 SAT
-
 Escherichia coli
2.3.1.30 SAT
-
 Arabidopsis thaliana
2.3.1.30 SAT
-
-
2.3.1.30 SAT
-
 Nicotiana tabacum