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Literature summary extracted from

  • Murillo, M.; Fogilia, R.; Diller, A.; Lee, S.; Leustek, T.
    Serine acetyltransferase from Arabidopsis thaliana can functionally complement the cysteine requirement of a cysE mutant strain of Escherichia coli (1996), Cell. Mol. Biol. Res., 41, 425-433.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.30 expression of SAT1 gene as glutathione-S-transferase fusion protein in Escherichia coli DH5alpha, complementation of serine acetyltransferase mutant Escherichia coli strain JM39, single copy gene, amino acid sequence determination Arabidopsis thaliana
2.3.1.30 SAT1 is closely linked to SAT5, high DNA sequence homology Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.30 acetyl-CoA + L-serine Arabidopsis thaliana
-
 CoA + O-acetyl-L-serine
-
 ?
2.3.1.30 acetyl-CoA + L-serine Arabidopsis thaliana regulatory role in cysteine biosynthesis CoA + O-acetyl-L-serine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.30 Arabidopsis thaliana Q39218 gene SAT1-6, mitochondrial isozyme
-
2.3.1.30 Arabidopsis thaliana Q42588 gene SAT1
-
2.3.1.30 Arabidopsis thaliana Q42588 gene SAT5
-
2.3.1.30 Escherichia coli P0A9D4
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.30 leaf SAT1 gene Arabidopsis thaliana
-
2.3.1.30 leaf SAT1-6 gene, major part Arabidopsis thaliana
-
2.3.1.30 root SAT1 gene Arabidopsis thaliana
-
2.3.1.30 root SAT1-6 gene, minor part Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.30 acetyl-CoA + L-serine
-
 Escherichia coli CoA + O-acetyl-L-serine
-
 ?
2.3.1.30 acetyl-CoA + L-serine
-
 Arabidopsis thaliana CoA + O-acetyl-L-serine
-
 ?
2.3.1.30 acetyl-CoA + L-serine regulatory role in cysteine biosynthesis Arabidopsis thaliana CoA + O-acetyl-L-serine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.30 ? x * 32000-35000, SDS-PAGE Escherichia coli
2.3.1.30 ? x * 32000-35000, SDS-PAGE Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.30 22
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.30 7.6
-
 assay at Arabidopsis thaliana