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Literature summary extracted from

  • Saito, K.; Yokoyama, H.; Noji, M.; Murakoshi, I.
    Molecular cloning and characterization of a plant serine acetyltransferase playing a regulatory role in cysteine biosynthesis from watermelon (1995), J. Biol. Chem., 270, 16321-16326.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.30 isolation of cDNA clone from Citrullus vulgaris genetic library by complementation of a Cys- Escherichia coli strain JM 39/5, single copy gene, DNA and amino acid sequence determination Citrullus lanatus subsp. vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.30 CoA
-
 Citrullus lanatus subsp. vulgaris
2.3.1.30 L-cysteine allosteric inhibition Citrullus lanatus subsp. vulgaris
2.3.1.30 additional information beta-(pyrazol-1-yl)-L-alanine has no inhibitory effect Citrullus lanatus subsp. vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.30 0.13
-
 acetyl-CoA
-
 Citrullus lanatus subsp. vulgaris
2.3.1.30 0.59
-
 L-serine
-
 Citrullus lanatus subsp. vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.30 additional information
-
 multi-enzyme complex is formed between serine acetyltransferase and cysteine synthase possibly leading to metabolic channeling Citrullus lanatus subsp. vulgaris
2.3.1.30 450000 500000 multienzyme complex of L-serine acetyltransferase and L-cysteine synthase, gel filtration Citrullus lanatus subsp. vulgaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.30 acetyl-CoA + L-serine Citrullus lanatus subsp. vulgaris regulatory role in cysteine biosynthesis CoA + O-acetyl-L-serine
-
 ir
2.3.1.30 additional information Citrullus lanatus subsp. vulgaris multienzyme complex is formed between serine acetyltransferase and cysteine synthase possibly leading to metabolic channeling ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.30 Citrullus lanatus subsp. vulgaris
-
 watermelon
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.30 hypocotyl
-
 Citrullus lanatus subsp. vulgaris
-
2.3.1.30 additional information accumulation in etiolated plants Citrullus lanatus subsp. vulgaris
-
2.3.1.30 seedling hypocotyl of etiolated seedlings Citrullus lanatus subsp. vulgaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.30 14.7
-
 recombinant protein Citrullus lanatus subsp. vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.30 acetyl-CoA + L-serine
-
 Citrullus lanatus subsp. vulgaris CoA + O-acetyl-L-serine
-
 ?
2.3.1.30 acetyl-CoA + L-serine regulatory role in cysteine biosynthesis Citrullus lanatus subsp. vulgaris CoA + O-acetyl-L-serine
-
 ir
2.3.1.30 additional information multienzyme complex is formed between serine acetyltransferase and cysteine synthase possibly leading to metabolic channeling Citrullus lanatus subsp. vulgaris ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.30 7.6 8 Tris-buffer Citrullus lanatus subsp. vulgaris

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.30 0.0037
-
 L-cysteine versus L-serine Citrullus lanatus subsp. vulgaris
2.3.1.30 0.0056
-
 L-cysteine versus acetyl-CoA Citrullus lanatus subsp. vulgaris
2.3.1.30 0.064
-
 CoA versus acetyl-CoA Citrullus lanatus subsp. vulgaris