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Literature summary extracted from

  • Fubara, B.; Eckenrode, F.; Tressel, T.; Davis, L.
    Purification and properties of aminoacetone synthetase from beef liver mitochondria (1986), J. Biol. Chem., 261, 12189-12196.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.29 glycerol, 10% v/v, stabilizes Bos taurus
2.3.1.29 glycine stabilizes Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.29 Glycine methyl ester inactivation Bos taurus
2.3.1.29 IAA
-
 Bos taurus
2.3.1.29 additional information no inhibition by DTNB Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.29 0.053
-
 acetyl-CoA
-
 Bos taurus
2.3.1.29 22
-
 glycine
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.29 mitochondrion
-
 Bos taurus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.29 28000
-
 2 * 28000, SDS-PAGE, 2 * 29165, calculated from amino acid composition Bos taurus
2.3.1.29 29165
-
 2 * 28000, SDS-PAGE, 2 * 29165, calculated from amino acid composition Bos taurus
2.3.1.29 56000
-
 gel filtration Bos taurus
2.3.1.29 58330
-
 calculated from amino acid composition Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.29 acetyl-CoA + glycine Bos taurus involved in L-threonine catabolism, inducible CoA + 2-amino-3-oxobutanoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.29 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.29
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.29 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.29 16.4
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.29 acetyl-CoA + aminomalonate
-
 Bos taurus CoA + CO2 + 2-amino-3-oxobutanoate same product as in reaction with glycine, aminomalonate is decarboxylated to glycine by the enzyme, in the absence of acetyl-CoA: glycine is the product ?
2.3.1.29 acetyl-CoA + glycine specific for glycine Bos taurus CoA + 2-amino-3-oxobutanoate spontaneous decarboxylation to aminoacetone r
2.3.1.29 acetyl-CoA + glycine succinyl-CoA Bos taurus CoA + 2-amino-3-oxobutanoate spontaneous decarboxylation to aminoacetone r
2.3.1.29 acetyl-CoA + glycine not: acetyl phosphate Bos taurus CoA + 2-amino-3-oxobutanoate spontaneous decarboxylation to aminoacetone r
2.3.1.29 acetyl-CoA + glycine no substrate: L-alanine Bos taurus CoA + 2-amino-3-oxobutanoate spontaneous decarboxylation to aminoacetone r
2.3.1.29 acetyl-CoA + glycine no substrate: glycine methylester Bos taurus CoA + 2-amino-3-oxobutanoate spontaneous decarboxylation to aminoacetone r
2.3.1.29 acetyl-CoA + glycine acetyl-CoA cannot be replaced by acetyl phosphate Bos taurus CoA + 2-amino-3-oxobutanoate spontaneous decarboxylation to aminoacetone r
2.3.1.29 acetyl-CoA + glycine involved in L-threonine catabolism, inducible Bos taurus CoA + 2-amino-3-oxobutanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.29 dimer 2 * 28000, SDS-PAGE, 2 * 29165, calculated from amino acid composition Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.29 30
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.29 additional information
-
 pI: 5.2 Bos taurus
2.3.1.29 7.5
-
-
 Bos taurus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.1.29 6.5 8 stable Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.29 pyridoxal 5'-phosphate requirement, stoichiometry: 1 mol per mol subunit Bos taurus