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Literature summary extracted from

  • Lee, R.G.; Willingham, M.C.; Davis, M.A.; Skinner, K.A.; Rudel, L.L.
    Differential expression of ACAT1 and ACAT2 among cells within liver, intestine, kidney, and adrenal of nonhuman primates (2000), J. Lipid Res., 41, 1991-2001.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.26 expression in AC29 cells Platyrrhini

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.26 endoplasmic reticulum ACAT2: rough Platyrrhini 5783
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.26 47000
-
 immunoblotting, ACAT2 enzyme Platyrrhini
2.3.1.26 53000
-
 immunoblotting, ACAT1 enzyme Platyrrhini

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.26 long-chain fatty acyl-CoA + cholesterol Platyrrhini
-
 CoA + cholesteryl long-chain fatty acyl ester
-
 ?
2.3.1.26 additional information Platyrrhini two closely related enzymes identified that catalyse the esterification of cholesterol using acyl-CoA substrates, namely ACAT1 and ACAT2, the orientation of ACAT2 in the endoplasmic reticulum membrane, in addition to its expression only in liver and intestine, suggests that this enzyme may have as a primary function, the secretion of cholesteryl esters into apolipoprotein B-containing lipoproteins ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.26 Platyrrhini
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.26 adrenal gland ACAT1: strongly present in the cells of the cortex Platyrrhini
-
2.3.1.26 glomerulus ACAT1, distal tubules and podocytes Platyrrhini
-
2.3.1.26 goblet cell ACAT1 Platyrrhini
-
2.3.1.26 hepatocyte two different forms of the enzyme are present, ACAT1 and ACAT2 Platyrrhini
-
2.3.1.26 intestine ACAT2: strongly present in the apical third of the mucosal cells, ACAT1: strong signal in goblet cells, Paneth cells and villus macrophages Platyrrhini
-
2.3.1.26 jejunum
-
 Platyrrhini
-
2.3.1.26 kidney
-
 Platyrrhini
-
2.3.1.26 Kupffer cell ACAT1 present Platyrrhini
-
2.3.1.26 liver
-
 Platyrrhini
-
2.3.1.26 macrophage ACAT1, villus Platyrrhini
-
2.3.1.26 paneth cell ACAT1 Platyrrhini
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.26 0.00015
-
 kidney microsomes Platyrrhini
2.3.1.26 0.00087
-
 small intestine microsomes Platyrrhini
2.3.1.26 0.00135
-
 liver microsomes Platyrrhini
2.3.1.26 0.0037
-
 adrenal microsomes Platyrrhini

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.26 long-chain fatty acyl-CoA + cholesterol
-
 Platyrrhini CoA + cholesteryl long-chain fatty acyl ester
-
 ?
2.3.1.26 additional information two closely related enzymes identified that catalyse the esterification of cholesterol using acyl-CoA substrates, namely ACAT1 and ACAT2, the orientation of ACAT2 in the endoplasmic reticulum membrane, in addition to its expression only in liver and intestine, suggests that this enzyme may have as a primary function, the secretion of cholesteryl esters into apolipoprotein B-containing lipoproteins Platyrrhini ?
-
 ?
2.3.1.26 oleoyl-CoA + cholesterol
-
 Platyrrhini CoA + cholesteryl oleate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.26 37
-
 assay at Platyrrhini