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Literature summary extracted from

  • Yu, C.; Kennedy, N.J.; Chang, C.C.Y.; Rothblatt, J.A.
    Molecular cloning and characterization of two isoforms of Saccharomyces cerevisiae acyl-CoA:sterol acyltransferase (1996), J. Biol. Chem., 271, 24157-24163.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.26 expression in sat1 sat2 mutant cells of Saccharomyces cerevisiae Homo sapiens
2.3.1.26 two genes, SAT1 and SAT2 encode isozymes of the enzyme Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.26 CI-976 cell-free extract of sat1 and sat2 cells: more than 95% inhibition at 0.2 mM, intact sat1 and sat2 cells: approximately 75-80% inhibition at 0.2 mM Homo sapiens
2.3.1.26 CI-976 cell-free extract of wild-type cells: approximately 90% inhibition at 0.1 mM and more than 95% inhibition at 0.2 mM, intact wild-type cells: approximately 75-80% inhibition at 0.2 mM Saccharomyces cerevisiae
2.3.1.26 DuP 128
-
 Homo sapiens
2.3.1.26 DuP 128 approximately 50% inhibition at 0.03-0.04 mM Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.26 52000
-
 recombinant enzyme Homo sapiens
2.3.1.26 71600
-
 deduced from amino acid sequence data Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.26 Homo sapiens
-
-
-
2.3.1.26 Saccharomyces cerevisiae P53629
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.26 glycoprotein there are six potential N-linked glycosylation sites in sat1, two of which are conserved in sat2 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.26 additional information
-
 when yeast-expressed enzyme activity is measured in the presence of ergosterol liposomes, specific activity is reduced more than 80%, recombinant enzyme is active but unable to esterify ergosterol efficiently Homo sapiens
2.3.1.26 0.0009
-
 recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.26 oleoyl-CoA + cholesterol
-
 Saccharomyces cerevisiae CoA + cholesteryl oleate
-
 ?
2.3.1.26 oleoyl-CoA + cholesterol recombinant enzyme Homo sapiens CoA + cholesteryl oleate
-
 ?
2.3.1.26 oleoyl-CoA + ergosterol endogenous ergosterol as substrate or exogenous ergosterol in the form of ergosterol/phosphatidylcholine vesicles as substrate Saccharomyces cerevisiae CoA + ergosteryl oleate
-
 ?
2.3.1.26 oleoyl-CoA + ergosterol recombinant enzyme: restores ergosteryl oleate formation in vivo to only approximately 8% of that catalysed by yeast enzyme in wild-type cells Homo sapiens CoA + ergosteryl oleate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.26 37
-
 assay at Homo sapiens
2.3.1.26 37
-
 assay at Saccharomyces cerevisiae