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Literature summary extracted from

  • Cheng, D.; Chang C.C.Y.; Qu, X.; Chang, T.
    Activation of acyl-coenzyme A:cholesterol acyltransferase by cholesterol or by oxysterol in a cell-free system (1995), J. Biol. Chem., 270, 685-695.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.26 25-hydroxycholesterol activates Homo sapiens
2.3.1.26 6-ketocholestanol added at high concentration causes modest increase in enzyme activity Homo sapiens
2.3.1.26 7-ketocholesterol added at high concentration causes modest increase in enzyme activity Homo sapiens
2.3.1.26 cholesterol serves as an enzyme activator in vitro, in addition to its role as an enzyme substrate Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.26 expression in insect SF9 cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.26 CI-976 inhibits Homo sapiens
2.3.1.26 DuP 128 inhibits Homo sapiens
2.3.1.26 octimibate inhibits Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.26 endoplasmic reticulum
-
 Homo sapiens 5783
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.26 Homo sapiens
-
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.3.1.26 solubilization of the cell extracts with deoxycholate followed by dilution into preformed liposomes Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.26 additional information
-
 activation of the enzyme by adding 25-hydroxycholesterol to intact cells or to cell-free extracts Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.26 oleoyl-CoA + cholesterol
-
 Homo sapiens CoA + cholesteryl oleate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.26 More SDS-PAGE: detection of two protein bands with apparent sizes at 50 and 56 kDa, immunoblots: existence of 100 and 200 kDa bands, clarification of these observations needed Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.26 37
-
 assay at Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.26 additional information
-
 enzyme activity expressed in Sf9 insect cells exhibits heat stability comparable of that of human enzyme, its heat stability is significantly greater than that of hamster enzyme Homo sapiens