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Literature summary extracted from

  • Kinnunen, P.M.; DeMichele, A.; Lange, L.G.
    Chemical modification of acyl-CoA:cholesterol O-acyltransferase. 1. Identification of acyl-CoA:cholesterol O-acyltransferase subtypes by differential diethyl pyrocarbonate sensitivity (1988), Biochemistry, 27, 7344-7350.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.26 Acetic anhydride 2 distinct tissue types with difference in reactivity, the inhibitor appears to modify a histidine residue Oryctolagus cuniculus
2.3.1.26 diethyl dicarbonate 2 distinct tissue types with difference in reactivity, DEP-sensitive subtype typified by aortic ACTAT, DEP-resistant subtype typified by liver ACAT, irreversible inhibition, the inhibitor appear to modify a histidine residue Oryctolagus cuniculus
2.3.1.26 p-mercuribenzoate 100% inhibition of liver and aortic enzyme at 1 mM Oryctolagus cuniculus
2.3.1.26 Phenylglyoxal concentration-dependent inhibition of liver enzyme with 65% inhibition at 5 mM Oryctolagus cuniculus
2.3.1.26 Tetranitromethane 100% inhibition of liver and aortic enzyme at 1 mM Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.26 microsome
-
 Oryctolagus cuniculus
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.26 Oryctolagus cuniculus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.26 a long-chain acyl-CoA + a sterol = CoA + a long-chain 3-hydroxysterol ester mechanism, a histidine is implicated in the catalytic mechanism of the enzyme Oryctolagus cuniculus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.26 adipose tissue
-
 Oryctolagus cuniculus
-
2.3.1.26 adrenal gland 1.7fold induction of activity after 60 days of cholesterol feeding Oryctolagus cuniculus
-
2.3.1.26 aorta 41fold induction of activity after 60 days of cholesterol feeding Oryctolagus cuniculus
-
2.3.1.26 brain 1.6fold induction of activity after 60 days of cholesterol feeding Oryctolagus cuniculus
-
2.3.1.26 heart 6fold induction of activity after 60 days of cholesterol feeding Oryctolagus cuniculus
-
2.3.1.26 intestine 23fold induction of activity after 60 days of cholesterol feeding Oryctolagus cuniculus
-
2.3.1.26 kidney
-
 Oryctolagus cuniculus
-
2.3.1.26 liver 27fold induction of activity after 60 days of cholesterol feeding Oryctolagus cuniculus
-
2.3.1.26 lung 3fold induction of activity after 60 days of cholesterol feeding Oryctolagus cuniculus
-
2.3.1.26 additional information overview: enzyme activity in normal and cholesterol-fed organs, identification of two distinct tissue types of the enzyme Oryctolagus cuniculus
-
2.3.1.26 pancreas 25fold induction of activity after 60 days of cholesterol feeding Oryctolagus cuniculus
-
2.3.1.26 spleen 7fold induction of activity after 60 days of cholesterol feeding Oryctolagus cuniculus
-
2.3.1.26 stomach
-
 Oryctolagus cuniculus
-
2.3.1.26 testis 1.8fold induction of activity after 60 days of cholesterol feeding Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.26 additional information
-
 overview: normal and cholesterol-fed organs Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.26 acyl-CoA + cholesterol
-
 Oryctolagus cuniculus CoA + cholesterol ester
-
 ?
2.3.1.26 oleoyl-CoA + cholesterol
-
 Oryctolagus cuniculus CoA + cholesteryl oleate
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.26 0.04
-
 diethyl dicarbonate aortic enzyme Oryctolagus cuniculus
2.3.1.26 0.5
-
 Acetic anhydride less than, aortic enzyme Oryctolagus cuniculus
2.3.1.26 1.5
-
 diethyl dicarbonate liver enzyme Oryctolagus cuniculus
2.3.1.26 5
-
 Acetic anhydride greater than, liver enzyme Oryctolagus cuniculus