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Literature summary extracted from

  • Hasegawa-Sasaki, H.; Ohno, K.
    Extraction and partial purification of acyl-CoA:1-acyl-sn-glycero-3-phosphocholine acyltransferase from rat liver microsomes (1980), Biochim. Biophys. Acta, 617, 205-217.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.23 methanol activation, arachidonoyl-CoA as substrate Rattus norvegicus

General Stability

EC Number General Stability Organism
2.3.1.23 glycerol, 50%, stabilizes Rattus norvegicus
2.3.1.23 low ionic strength stabilizes during purification Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.23 Brij 35 0.1% Rattus norvegicus
2.3.1.23 Brij 98 0.1% Rattus norvegicus
2.3.1.23 Lubrol WX 0.1% Rattus norvegicus
2.3.1.23 methanol oleoyl-CoA as substrate Rattus norvegicus
2.3.1.23 additional information not inhibited by IAA and DTNB Rattus norvegicus
2.3.1.23 N-ethylmaleimide 1 mM, 30% inhibition after 5 min, 95% inhibition after 45 min Rattus norvegicus
2.3.1.23 p-chloromercuribenzoate partially restorable by dithiothreitol Rattus norvegicus
2.3.1.23 p-chloromercuriphenyl sulfonic acid partially restorable by dithitothreitol Rattus norvegicus
2.3.1.23 Triton X-100 0.1% Rattus norvegicus
2.3.1.23 Tween 20 0.1% Rattus norvegicus
2.3.1.23 Tween 80 0.1% Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.23 0.0027
-
 palmitoyl-CoA
-
 Rattus norvegicus
2.3.1.23 0.0028
-
 oleoyl-CoA
-
 Rattus norvegicus
2.3.1.23 0.0032
-
 Arachidonoyl-CoA
-
 Rattus norvegicus
2.3.1.23 0.0035
-
 linoleoyl-CoA
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.23 microsome
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.23 225000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.23 Rattus norvegicus
-
 male Wistar
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.23 partially purified Rattus norvegicus
2.3.1.23 Ultrogel AcA 34 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.23 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.23 2.3
-
 acyl donor arachidonoyl-CoA Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.3.1.23 -20°C, multiple freeze-thawing cycles, 6 weeks, 20% loss of activity Rattus norvegicus
2.3.1.23 -20°C, partially purified enzyme, several months Rattus norvegicus
2.3.1.23 -20°C, purified enzyme, at least 3 weeks Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.23 acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
-
 Rattus norvegicus CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
 r
2.3.1.23 arachidonoyl-CoA + 1-acyl-sn-glycero-3-phosphoethanolamine
-
 Rattus norvegicus CoA + 1-acyl-2-arachidonoyl-sn-glycero-3-phosphoethanolamine
-
 ?
2.3.1.23 arachidonoyl-CoA + 1-acyl-sn-glycero-3-phosphoinositol
-
 Rattus norvegicus CoA + 1-acyl-2-arachidonoyl-sn-glycero-3-phosphoinositol
-
 ?
2.3.1.23 arachidonoyl-CoA + 1-acyl-sn-glycero-3-phosphoserine
-
 Rattus norvegicus CoA + 1-acyl-2-arachidonoyl-sn-glycero-3-phosphoserine
-
 ?
2.3.1.23 linoleoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
-
 Rattus norvegicus CoA + 1-acyl-2-linoleoyl-sn-glycero-3-phosphocholine
-
 ?
2.3.1.23 oleoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
-
 Rattus norvegicus CoA + 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.23 25
-
 assay at Rattus norvegicus
2.3.1.23 30
-
-
 Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.23 37
-
 oleoyl-CoA specific activity loses 50% of activity after 90 min, arachidonoyl-CoA specific activity loses 45% of activity after 90 min Rattus norvegicus
2.3.1.23 45
-
 10% loss of activity after 1 min Rattus norvegicus
2.3.1.23 48
-
 half-life: 1 min, oleoyl-CoA specific activity Rattus norvegicus
2.3.1.23 50
-
 50% loss of activity after 1 min Rattus norvegicus
2.3.1.23 52
-
 half-life: 1 min, arachidonoyl-CoA specific activity Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.23 8
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.23 6 9 approx. half-maximal activity at pH 6.0 and 9.6 Rattus norvegicus