BRENDA - Enzyme Database

Factors affecting the acyl selectivities of acyltransferases in Escherichia coli

Okuyama, H.; Yamada, K.; Ikezawa, H.; Wakil, S.J.; J. Biol. Chem. 251, 2487-2492 (1976)

Data extracted from this reference:

Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.15
plasma membrane
-
Escherichia coli
5886
-
2.3.1.51
membrane
-
Escherichia coli
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.1.15
KCl
-
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.15
Escherichia coli
-
B
-
2.3.1.15
Escherichia coli B / ATCC 11303
-
B
-
2.3.1.51
Escherichia coli
-
B
-
2.3.1.51
Escherichia coli B / ATCC 11303
-
B
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
saturated and unsaturated acyl-CoAs are substrates
486374
Escherichia coli
CoA + 1-acyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli
?
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
exclusively acylates glycerol 3-phosphate at the C-1-position
486374
Escherichia coli
CoA + 1-acyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli
?
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
saturated and unsaturated acyl-CoAs are substrates
486374
Escherichia coli B / ATCC 11303
CoA + 1-acyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli B / ATCC 11303
?
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
exclusively acylates glycerol 3-phosphate at the C-1-position
486374
Escherichia coli B / ATCC 11303
CoA + 1-acyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli B / ATCC 11303
?
2.3.1.15
myristoyl-CoA + sn-glycerol 3-phosphate
i.e. tetradecanoyl-CoA
486374
Escherichia coli
CoA + 1-myristoyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli
?
2.3.1.15
myristoyl-CoA + sn-glycerol 3-phosphate
i.e. tetradecanoyl-CoA
486374
Escherichia coli B / ATCC 11303
CoA + 1-myristoyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli B / ATCC 11303
?
2.3.1.15
n-dodecanoyl-CoA + sn-glycerol 3-phosphate
i.e. lauroyl-CoA, poor substrate
486374
Escherichia coli
CoA + 1-n-dodecanoyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli
?
2.3.1.15
n-dodecanoyl-CoA + sn-glycerol 3-phosphate
i.e. lauroyl-CoA, poor substrate
486374
Escherichia coli B / ATCC 11303
CoA + 1-n-dodecanoyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli B / ATCC 11303
?
2.3.1.15
oleoyl-CoA + sn-glycerol 3-phosphate
-
486374
Escherichia coli
CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli
?
2.3.1.15
palmitoleoyl-CoA + sn-glycerol 3-phosphate
-
486374
Escherichia coli
CoA + 1-palmitoleoyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli
?
2.3.1.15
palmitoyl-CoA + sn-glycerol 3-phosphate
i.e. n-hexadecanoyl-CoA, best substrate
486374
Escherichia coli
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli
?
2.3.1.15
palmitoyl-CoA + sn-glycerol 3-phosphate
i.e. n-hexadecanoyl-CoA, best substrate
486374
Escherichia coli B / ATCC 11303
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli B / ATCC 11303
?
2.3.1.15
stearoyl-CoA + sn-glycerol 3-phosphate
-
486374
Escherichia coli
CoA + 1-stearoyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli
?
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
acyl-donors are saturated and unsaturated fatty acyl-CoAs
486374
Escherichia coli
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli
?
2.3.1.51
lauroyl-CoA + 1-acyl-sn-glycerol 3-phosphate
lauroyl-CoA is a poor substrate
486374
Escherichia coli
CoA + 1-acyl-2-lauroyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli
?
2.3.1.51
additional information
the enzyme can utilize various saturated and unsaturated acyl-CoAs at comparable rates, unsaturated fatty acyl-CoAs with cis- or trans-configuration are slightly better substrates than saturated acyl-CoAs
486374
Escherichia coli
?
-
-
-
-
2.3.1.51
additional information
effect of variable ratios of oleoyl-CoA to palmitoyl-CoA on the reaction
486374
Escherichia coli
?
-
-
-
-
2.3.1.51
myristoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
myristoyl-CoA is a poor substrate
486374
Escherichia coli
CoA + 1-acyl-2-myristoyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli
?
2.3.1.51
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
high selectivity for oleate
486374
Escherichia coli
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli
?
2.3.1.51
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
palmitoyl-CoA is the best substrate
486374
Escherichia coli
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.51
23
26
assay at
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.51
7.5
-
assay at
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.15
plasma membrane
-
Escherichia coli
5886
-
2.3.1.51
membrane
-
Escherichia coli
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.1.15
KCl
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
saturated and unsaturated acyl-CoAs are substrates
486374
Escherichia coli
CoA + 1-acyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli
?
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
exclusively acylates glycerol 3-phosphate at the C-1-position
486374
Escherichia coli
CoA + 1-acyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli
?
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
saturated and unsaturated acyl-CoAs are substrates
486374
Escherichia coli B / ATCC 11303
CoA + 1-acyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli B / ATCC 11303
?
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
exclusively acylates glycerol 3-phosphate at the C-1-position
486374
Escherichia coli B / ATCC 11303
CoA + 1-acyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli B / ATCC 11303
?
2.3.1.15
myristoyl-CoA + sn-glycerol 3-phosphate
i.e. tetradecanoyl-CoA
486374
Escherichia coli
CoA + 1-myristoyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli
?
2.3.1.15
myristoyl-CoA + sn-glycerol 3-phosphate
i.e. tetradecanoyl-CoA
486374
Escherichia coli B / ATCC 11303
CoA + 1-myristoyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli B / ATCC 11303
?
2.3.1.15
n-dodecanoyl-CoA + sn-glycerol 3-phosphate
i.e. lauroyl-CoA, poor substrate
486374
Escherichia coli
CoA + 1-n-dodecanoyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli
?
2.3.1.15
n-dodecanoyl-CoA + sn-glycerol 3-phosphate
i.e. lauroyl-CoA, poor substrate
486374
Escherichia coli B / ATCC 11303
CoA + 1-n-dodecanoyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli B / ATCC 11303
?
2.3.1.15
oleoyl-CoA + sn-glycerol 3-phosphate
-
486374
Escherichia coli
CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli
?
2.3.1.15
palmitoleoyl-CoA + sn-glycerol 3-phosphate
-
486374
Escherichia coli
CoA + 1-palmitoleoyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli
?
2.3.1.15
palmitoyl-CoA + sn-glycerol 3-phosphate
i.e. n-hexadecanoyl-CoA, best substrate
486374
Escherichia coli
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli
?
2.3.1.15
palmitoyl-CoA + sn-glycerol 3-phosphate
i.e. n-hexadecanoyl-CoA, best substrate
486374
Escherichia coli B / ATCC 11303
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli B / ATCC 11303
?
2.3.1.15
stearoyl-CoA + sn-glycerol 3-phosphate
-
486374
Escherichia coli
CoA + 1-stearoyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli
?
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
acyl-donors are saturated and unsaturated fatty acyl-CoAs
486374
Escherichia coli
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli
?
2.3.1.51
lauroyl-CoA + 1-acyl-sn-glycerol 3-phosphate
lauroyl-CoA is a poor substrate
486374
Escherichia coli
CoA + 1-acyl-2-lauroyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli
?
2.3.1.51
additional information
the enzyme can utilize various saturated and unsaturated acyl-CoAs at comparable rates, unsaturated fatty acyl-CoAs with cis- or trans-configuration are slightly better substrates than saturated acyl-CoAs
486374
Escherichia coli
?
-
-
-
-
2.3.1.51
additional information
effect of variable ratios of oleoyl-CoA to palmitoyl-CoA on the reaction
486374
Escherichia coli
?
-
-
-
-
2.3.1.51
myristoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
myristoyl-CoA is a poor substrate
486374
Escherichia coli
CoA + 1-acyl-2-myristoyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli
?
2.3.1.51
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
high selectivity for oleate
486374
Escherichia coli
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli
?
2.3.1.51
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
palmitoyl-CoA is the best substrate
486374
Escherichia coli
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
-
486374
Escherichia coli
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.51
23
26
assay at
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.51
7.5
-
assay at
Escherichia coli