BRENDA - Enzyme Database

Positional specificities of acyl coenzyme A: glycerophosphate and acyl coenzyme A: monoacylglycerophosphate acyltransferases in Escherichia coli

Okuyama, H.; Wakil, S.J.; J. Biol. Chem. 248, 5197-5205 (1973)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.3.1.51
slow freezing inactivates
Escherichia coli
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.51
MgCl2
at high concentrations
Escherichia coli
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.15
plasma membrane
-
Escherichia coli
5886
-
2.3.1.51
membrane
-
Escherichia coli
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.1.51
additional information
no Mg2+-requirement
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.15
Escherichia coli
-
B
-
2.3.1.15
Escherichia coli B / ATCC 11303
-
B
-
2.3.1.51
Escherichia coli
-
B
-
2.3.1.51
Escherichia coli B / ATCC 11303
-
B
-
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.51
-15°C to 4°C, particulate preparation, fast-frozen in liquid N2, unstable
Escherichia coli
2.3.1.51
-15°C, less than a day
Escherichia coli
2.3.1.51
-80°C, particulate preparation, fast-frozen in liquid N2, 3 months
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
exclusively acylates glycerol 3-phosphate at the C-1-position
486373
Escherichia coli
CoA + 1-acyl-sn-glycerol 3-phosphate
-
-
-
?
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
exclusively acylates glycerol 3-phosphate at the C-1-position
486373
Escherichia coli B / ATCC 11303
CoA + 1-acyl-sn-glycerol 3-phosphate
-
-
-
?
2.3.1.15
oleoyl-CoA + sn-glycerol 3-phosphate
-
486373
Escherichia coli
CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
486373
Escherichia coli
?
2.3.1.15
oleoyl-CoA + sn-glycerol 3-phosphate
-
486373
Escherichia coli B / ATCC 11303
CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
486373
Escherichia coli B / ATCC 11303
?
2.3.1.15
palmitoyl-CoA + sn-glycerol 3-phosphate
-
486373
Escherichia coli
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
486373
Escherichia coli
?
2.3.1.15
palmitoyl-CoA + sn-glycerol 3-phosphate
-
486373
Escherichia coli B / ATCC 11303
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
486373
Escherichia coli B / ATCC 11303
?
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
486373
Escherichia coli
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
i.e. phosphatidic acid
486373
Escherichia coli
?
2.3.1.51
acyl-CoA + 2-acyl-sn-glycerol 3-phosphate
the enzyme does not acylate 2-acyl-sn-glycerol 3-phosphate
486373
Escherichia coli
CoA + ?
-
-
-
-
2.3.1.51
additional information
effect of acyl-CoA concentrations on enzyme reaction
486373
Escherichia coli
?
-
-
-
-
2.3.1.51
additional information
1-acyl-sn-glycerol 3-phosphate is readily acylated by unsaturated and saturated acyl-CoAs
486373
Escherichia coli
?
-
-
-
-
2.3.1.51
additional information
effect of acyl-CoA concentrations on enzyme reaction
486373
Escherichia coli B / ATCC 11303
?
-
-
-
-
2.3.1.51
additional information
1-acyl-sn-glycerol 3-phosphate is readily acylated by unsaturated and saturated acyl-CoAs
486373
Escherichia coli B / ATCC 11303
?
-
-
-
-
2.3.1.51
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
twice as good as palmitoyl-CoA
486373
Escherichia coli
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
-
486373
Escherichia coli
?
2.3.1.51
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
486373
Escherichia coli
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
-
486373
Escherichia coli
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.51
7.5
8
-
Escherichia coli
2.3.1.51
7.5
-
assay at
Escherichia coli
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.51
6.6
8.6
about half-maximal activity at pH 6.6 and 8.6
Escherichia coli
General Stability (protein specific)
EC Number
General Stability
Organism
2.3.1.51
slow freezing inactivates
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.51
MgCl2
at high concentrations
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.15
plasma membrane
-
Escherichia coli
5886
-
2.3.1.51
membrane
-
Escherichia coli
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.1.51
additional information
no Mg2+-requirement
Escherichia coli
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.51
-15°C to 4°C, particulate preparation, fast-frozen in liquid N2, unstable
Escherichia coli
2.3.1.51
-15°C, less than a day
Escherichia coli
2.3.1.51
-80°C, particulate preparation, fast-frozen in liquid N2, 3 months
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
exclusively acylates glycerol 3-phosphate at the C-1-position
486373
Escherichia coli
CoA + 1-acyl-sn-glycerol 3-phosphate
-
-
-
?
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
exclusively acylates glycerol 3-phosphate at the C-1-position
486373
Escherichia coli B / ATCC 11303
CoA + 1-acyl-sn-glycerol 3-phosphate
-
-
-
?
2.3.1.15
oleoyl-CoA + sn-glycerol 3-phosphate
-
486373
Escherichia coli
CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
486373
Escherichia coli
?
2.3.1.15
oleoyl-CoA + sn-glycerol 3-phosphate
-
486373
Escherichia coli B / ATCC 11303
CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
486373
Escherichia coli B / ATCC 11303
?
2.3.1.15
palmitoyl-CoA + sn-glycerol 3-phosphate
-
486373
Escherichia coli
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
486373
Escherichia coli
?
2.3.1.15
palmitoyl-CoA + sn-glycerol 3-phosphate
-
486373
Escherichia coli B / ATCC 11303
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
486373
Escherichia coli B / ATCC 11303
?
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
486373
Escherichia coli
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
i.e. phosphatidic acid
486373
Escherichia coli
?
2.3.1.51
acyl-CoA + 2-acyl-sn-glycerol 3-phosphate
the enzyme does not acylate 2-acyl-sn-glycerol 3-phosphate
486373
Escherichia coli
CoA + ?
-
-
-
-
2.3.1.51
additional information
effect of acyl-CoA concentrations on enzyme reaction
486373
Escherichia coli
?
-
-
-
-
2.3.1.51
additional information
1-acyl-sn-glycerol 3-phosphate is readily acylated by unsaturated and saturated acyl-CoAs
486373
Escherichia coli
?
-
-
-
-
2.3.1.51
additional information
effect of acyl-CoA concentrations on enzyme reaction
486373
Escherichia coli B / ATCC 11303
?
-
-
-
-
2.3.1.51
additional information
1-acyl-sn-glycerol 3-phosphate is readily acylated by unsaturated and saturated acyl-CoAs
486373
Escherichia coli B / ATCC 11303
?
-
-
-
-
2.3.1.51
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
twice as good as palmitoyl-CoA
486373
Escherichia coli
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
-
486373
Escherichia coli
?
2.3.1.51
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
486373
Escherichia coli
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
-
486373
Escherichia coli
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.51
7.5
8
-
Escherichia coli
2.3.1.51
7.5
-
assay at
Escherichia coli
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.51
6.6
8.6
about half-maximal activity at pH 6.6 and 8.6
Escherichia coli