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Literature summary extracted from

  • Schlossman, D.M.; Bell, R.M.
    Glycerolipid biosynthesis in Saccharomyces cerevisiae: sn-glycerol-3-phosphate and dihydroxyacetone phosphate acyltransferase activities (1978), J. Bacteriol., 133, 1368-1376.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.15 Triton X-100 i.e. octylphenoxy polyethoxyethanol, slight stimulation at 0.1 mg/ml Saccharomyces cerevisiae
2.3.1.42 Triton X-100 slight stimulation Saccharomyces cerevisiae
2.3.1.42 Triton X-100 0.1 mg/ml Saccharomyces cerevisiae
2.3.1.42 Triton X-100 inhibition above 0.1 mg/ml Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.15 deoxycholate above 0.35 mg/ml Saccharomyces cerevisiae
2.3.1.15 dihydroxyacetone phosphate
-
 Saccharomyces cerevisiae
2.3.1.15 N-ethylmaleimide 0.4 mM, 60% inhibition after 10 min Saccharomyces cerevisiae
2.3.1.15 palmitoyl-CoA 0.08 mM Saccharomyces cerevisiae
2.3.1.15 Triton X-100 strong, above 0.15 mg/ml Saccharomyces cerevisiae
2.3.1.15 Trypsin 0.06 mg/ml, 80% inhibition after 10 min Saccharomyces cerevisiae
2.3.1.42 glycerol 3-phosphate competitive to dihydroxyacetone phosphate Saccharomyces cerevisiae
2.3.1.42 N-ethylmaleimide not peroxisomal enzyme Saccharomyces cerevisiae
2.3.1.42 Triton X-100 0.1 mg/ml; above 0.1 mg/ml Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.15 0.03
-
 sn-glycerol 3-phosphate
-
 Saccharomyces cerevisiae
2.3.1.42 0.79
-
 dihydroxyacetone phosphate
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.42 membrane
-
 Saccharomyces cerevisiae 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.42 palmitoyl-CoA + dihydroxyacetone phosphate Saccharomyces cerevisiae initiates glycerolipid, especially ether-linked glycerolipid, biosynthesis in higher eukaryotes CoA + monopalmitoyldihydroxyacetone phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.15 Saccharomyces cerevisiae
-
 S288c
-
2.3.1.42 Saccharomyces cerevisiae
-
 S288c
-

Storage Stability

EC Number Storage Stability Organism
2.3.1.15 -15°C, 3-5 mg/ml protein, membrane preparation, at least 2 months Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate enzyme has both 3-glycerophosphate acyltransferase and dihydroxyacetonephosphate acyltransferase activity Saccharomyces cerevisiae CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 myristoyl-CoA + sn-glycerol 3-phosphate i.e. tetradecanoyl-CoA Saccharomyces cerevisiae CoA + 1-myristoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 n-dodecanoyl-CoA + sn-glycerol 3-phosphate i.e. lauroyl-CoA, poor substrate Saccharomyces cerevisiae CoA + 1-n-dodecanoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 oleoyl-CoA + sn-glycerol 3-phosphate
-
 Saccharomyces cerevisiae CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 palmitoyl-CoA + sn-glycerol 3-phosphate i.e. n-hexadecanoyl-CoA, best substrate Saccharomyces cerevisiae CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.42 acyl-CoA + dihydroxyacetone phosphate no acyl-donors are C4-C10-acyl-CoAs Saccharomyces cerevisiae CoA + acyldihydroxyacetone phosphate i.e. acylglycerone phosphate ?
2.3.1.42 dodecanoyl-CoA + dihydroxyacetone phosphate i.e. lauroyl-CoA, poor substrate Saccharomyces cerevisiae CoA + dodecanoyldihydroxyacetone phosphate
-
 ?
2.3.1.42 oleoyl-CoA + dihydroxyacetone phosphate poor substrate Saccharomyces cerevisiae CoA + oleoyldihydroxyacetone phosphate
-
 ?
2.3.1.42 palmitoyl-CoA + dihydroxyacetone phosphate
-
 Saccharomyces cerevisiae CoA + monopalmitoyldihydroxyacetone phosphate
-
 ?
2.3.1.42 palmitoyl-CoA + dihydroxyacetone phosphate initiates glycerolipid, especially ether-linked glycerolipid, biosynthesis in higher eukaryotes Saccharomyces cerevisiae CoA + monopalmitoyldihydroxyacetone phosphate
-
 ?
2.3.1.42 stearoyl-CoA + dihydroxyacetone phosphate
-
 Saccharomyces cerevisiae CoA + stearoyldihydroxyacetone phosphate
-
 ?
2.3.1.42 tetradecanoyl-CoA + dihydroxyacetone phosphate i.e. myristoyl-CoA Saccharomyces cerevisiae CoA + tetradecanoyldihydroxyacetone phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.15 More enzyme may be identical with EC 2.3.1.42 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.42 23
-
 assay at Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.42 48
-
 t1/2: 4 min Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.15 6.6 7
-
 Saccharomyces cerevisiae
2.3.1.15 7
-
 50% of maximal activity at pH 7.4 Saccharomyces cerevisiae
2.3.1.42 7
-
-
 Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.15 6.6 8.9 maximal activity at pH 6.6, approx. 30% of maximal activity at pH 8.9 Saccharomyces cerevisiae
2.3.1.42 7.4
-
 about half-maximal activity above Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.15 0.54
-
 dihydroxyacetone phosphate
-
 Saccharomyces cerevisiae
2.3.1.42 0.07
-
 glycerol 3-phosphate
-
 Saccharomyces cerevisiae