BRENDA - Enzyme Database

Enzymes of triacylglycerol formation in mammals

Bell, R.M.; Coleman, R.A.; The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ) 16, 87-111 (1983)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.15
Polymyxin B
activation of mitochondrial enzyme, inhibition of microsomal enzyme
Rattus norvegicus
General Stability
EC Number
General Stability
Organism
2.3.1.15
phospholipids stabilize
Escherichia coli
2.3.1.15
phospholipids stabilize
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.15
5,5'-dithiobis(2-nitro-benzoate)
-
Escherichia coli
2.3.1.15
5,5'-dithiobis(2-nitro-benzoate)
inhibition of microsomal enzyme, mitochondrial enzyme is not inhibited
Rattus norvegicus
2.3.1.15
acetone
inhibition of microsomal enzyme
Rattus norvegicus
2.3.1.15
iodoacetamide
strong inhibition of microsomal enzyme, mitochondrial enzyme is not inhibited
Rattus norvegicus
2.3.1.15
MgCl2
inhibition of microsomal enzyme
Rattus norvegicus
2.3.1.15
N-ethylmaleimide
-
Escherichia coli
2.3.1.15
N-ethylmaleimide
strong inhibition of microsomal isozyme, mitochondrial enzyme is not inhibited
Rattus norvegicus
2.3.1.15
Polymyxin B
0.5 mg/ml, approx. 75% inhibition of microsomal enzyme, slight stimulation of mitochondrial enzyme
Rattus norvegicus
2.3.1.15
Triton X-100
-
Rattus norvegicus
2.3.1.15
Triton X-100
-
Escherichia coli
2.3.1.42
glycerol 3-phosphate
adipocyte
Rattus norvegicus
2.3.1.42
N-ethylmaleimide
not peroxisomal enzyme
Rattus norvegicus
2.3.1.42
N-ethylmaleimide
not peroxisomal enzyme
Oryctolagus cuniculus
2.3.1.51
additional information
no inhibition by sulfhydryl reagents, 1-acylglycerol 3-phosphoethanol, 1-acylglycerol 3-phosphoethanolamine, 1-acylglycerol 3-phosphodimethylethanolamine, 1-acylglycerol 3-choline
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.15
microsome
membrane-bound
Rattus norvegicus
-
-
2.3.1.15
mitochondrial membrane
membrane-bound
Rattus norvegicus
31966
-
2.3.1.20
microsome
active site on cytoplasmic surface
Rattus norvegicus
-
-
2.3.1.42
microsome
-
Oryctolagus cuniculus
-
-
2.3.1.42
microsome
-
Rattus norvegicus
-
-
2.3.1.42
mitochondrion
outer membrane
Rattus norvegicus
5739
-
2.3.1.42
peroxisome
-
Oryctolagus cuniculus
5777
-
2.3.1.42
peroxisome
-
Rattus norvegicus
5777
-
2.3.1.51
microsome
intrinsic membrane protein, cytoplasmic surface; not rat liver peroxisomes
Rattus norvegicus
-
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.20
acyl-CoA + 1,2-diacylglycerol
Rattus norvegicus
last reaction in triacylglycerol synthesis
CoA + triacylglycerol
-
-
?
2.3.1.42
palmitoyl-CoA + dihydroxyacetone phosphate
Rattus norvegicus
peroxisomal enzyme involved in ether lipid biosynthesis
CoA + monopalmitoyldihydroxyacetone phosphate
-
-
?
2.3.1.42
palmitoyl-CoA + dihydroxyacetone phosphate
Oryctolagus cuniculus
peroxisomal enzyme involved in ether lipid biosynthesis
CoA + monopalmitoyldihydroxyacetone phosphate
-
-
?
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
Rattus norvegicus
involved in phospholipid biosynthesis
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
Rattus norvegicus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.15
Escherichia coli
-
-
-
2.3.1.15
Rattus norvegicus
-
-
-
2.3.1.20
Rattus norvegicus
-
-
-
2.3.1.42
Oryctolagus cuniculus
-
-
-
2.3.1.42
Rattus norvegicus
-
-
-
2.3.1.51
Rattus norvegicus
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
2.3.1.15
side-chain modification
activity of microsomal enzyme is probably regulated by phosphorylation-dephosphorylation
Rattus norvegicus
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.15
Triton X-100-solubilized
Escherichia coli
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.15
liver
-
Rattus norvegicus
-
2.3.1.15
mammary gland
lactating
Rattus norvegicus
-
2.3.1.15
additional information
distribution, overview
Escherichia coli
-
2.3.1.15
additional information
distribution, overview
Rattus norvegicus
-
2.3.1.20
adipocyte
-
Rattus norvegicus
-
2.3.1.20
intestinal mucosa
-
Rattus norvegicus
-
2.3.1.20
liver
-
Rattus norvegicus
-
2.3.1.20
mammary gland
-
Rattus norvegicus
-
2.3.1.20
additional information
distribution, overview
Rattus norvegicus
-
2.3.1.42
adipocyte
-
Rattus norvegicus
-
2.3.1.42
additional information
distribution, overview
Oryctolagus cuniculus
-
2.3.1.42
additional information
distribution, overview
Rattus norvegicus
-
2.3.1.51
liver
-
Rattus norvegicus
-
2.3.1.51
lung
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
saturated acyl-CoAs preferred
486338
Escherichia coli
CoA + 1-acyl-sn-glycerol 3-phosphate
-
-
-
?
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
saturated acyl-CoAs preferred
486338
Rattus norvegicus
CoA + 1-acyl-sn-glycerol 3-phosphate
-
-
-
?
2.3.1.20
acyl-CoA + 1,2-diacylglycerol
last reaction in triacylglycerol synthesis
486338
Rattus norvegicus
CoA + triacylglycerol
-
-
-
?
2.3.1.42
acyl-CoA + dihydroxyacetone phosphate
-
486338
Rattus norvegicus
CoA + acyldihydroxyacetone phosphate
i.e. acylglycerone phosphate
486338
Rattus norvegicus
?
2.3.1.42
acyl-CoA + dihydroxyacetone phosphate
-
486338
Oryctolagus cuniculus
CoA + acyldihydroxyacetone phosphate
i.e. acylglycerone phosphate
486338
Oryctolagus cuniculus
?
2.3.1.42
palmitoyl-CoA + dihydroxyacetone phosphate
peroxisomal enzyme involved in ether lipid biosynthesis
486338
Rattus norvegicus
CoA + monopalmitoyldihydroxyacetone phosphate
-
-
-
?
2.3.1.42
palmitoyl-CoA + dihydroxyacetone phosphate
peroxisomal enzyme involved in ether lipid biosynthesis
486338
Oryctolagus cuniculus
CoA + monopalmitoyldihydroxyacetone phosphate
-
-
-
?
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
486338
Rattus norvegicus
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
i.e. phosphatidic acid
486338
Rattus norvegicus
?
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
involved in phospholipid biosynthesis
486338
Rattus norvegicus
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
486338
Rattus norvegicus
?
2.3.1.51
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
486338
Rattus norvegicus
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
-
486338
Rattus norvegicus
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.42
5.5
-
membrane-bound peroxisomal enzyme
Oryctolagus cuniculus
2.3.1.42
5.5
-
membrane-bound peroxisomal enzyme
Rattus norvegicus
2.3.1.42
7.5
-
microsomal enzyme
Oryctolagus cuniculus
2.3.1.42
7.5
-
microsomal enzyme
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.15
Polymyxin B
activation of mitochondrial enzyme, inhibition of microsomal enzyme
Rattus norvegicus
General Stability (protein specific)
EC Number
General Stability
Organism
2.3.1.15
phospholipids stabilize
Escherichia coli
2.3.1.15
phospholipids stabilize
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.15
5,5'-dithiobis(2-nitro-benzoate)
-
Escherichia coli
2.3.1.15
5,5'-dithiobis(2-nitro-benzoate)
inhibition of microsomal enzyme, mitochondrial enzyme is not inhibited
Rattus norvegicus
2.3.1.15
acetone
inhibition of microsomal enzyme
Rattus norvegicus
2.3.1.15
iodoacetamide
strong inhibition of microsomal enzyme, mitochondrial enzyme is not inhibited
Rattus norvegicus
2.3.1.15
MgCl2
inhibition of microsomal enzyme
Rattus norvegicus
2.3.1.15
N-ethylmaleimide
-
Escherichia coli
2.3.1.15
N-ethylmaleimide
strong inhibition of microsomal isozyme, mitochondrial enzyme is not inhibited
Rattus norvegicus
2.3.1.15
Polymyxin B
0.5 mg/ml, approx. 75% inhibition of microsomal enzyme, slight stimulation of mitochondrial enzyme
Rattus norvegicus
2.3.1.15
Triton X-100
-
Rattus norvegicus
2.3.1.15
Triton X-100
-
Escherichia coli
2.3.1.42
glycerol 3-phosphate
adipocyte
Rattus norvegicus
2.3.1.42
N-ethylmaleimide
not peroxisomal enzyme
Rattus norvegicus
2.3.1.42
N-ethylmaleimide
not peroxisomal enzyme
Oryctolagus cuniculus
2.3.1.51
additional information
no inhibition by sulfhydryl reagents, 1-acylglycerol 3-phosphoethanol, 1-acylglycerol 3-phosphoethanolamine, 1-acylglycerol 3-phosphodimethylethanolamine, 1-acylglycerol 3-choline
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.15
microsome
membrane-bound
Rattus norvegicus
-
-
2.3.1.15
mitochondrial membrane
membrane-bound
Rattus norvegicus
31966
-
2.3.1.20
microsome
active site on cytoplasmic surface
Rattus norvegicus
-
-
2.3.1.42
microsome
-
Oryctolagus cuniculus
-
-
2.3.1.42
microsome
-
Rattus norvegicus
-
-
2.3.1.42
mitochondrion
outer membrane
Rattus norvegicus
5739
-
2.3.1.42
peroxisome
-
Oryctolagus cuniculus
5777
-
2.3.1.42
peroxisome
-
Rattus norvegicus
5777
-
2.3.1.51
microsome
intrinsic membrane protein, cytoplasmic surface; not rat liver peroxisomes
Rattus norvegicus
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.20
acyl-CoA + 1,2-diacylglycerol
Rattus norvegicus
last reaction in triacylglycerol synthesis
CoA + triacylglycerol
-
-
?
2.3.1.42
palmitoyl-CoA + dihydroxyacetone phosphate
Rattus norvegicus
peroxisomal enzyme involved in ether lipid biosynthesis
CoA + monopalmitoyldihydroxyacetone phosphate
-
-
?
2.3.1.42
palmitoyl-CoA + dihydroxyacetone phosphate
Oryctolagus cuniculus
peroxisomal enzyme involved in ether lipid biosynthesis
CoA + monopalmitoyldihydroxyacetone phosphate
-
-
?
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
Rattus norvegicus
involved in phospholipid biosynthesis
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
Rattus norvegicus
?
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
2.3.1.15
side-chain modification
activity of microsomal enzyme is probably regulated by phosphorylation-dephosphorylation
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.15
Triton X-100-solubilized
Escherichia coli
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.15
liver
-
Rattus norvegicus
-
2.3.1.15
mammary gland
lactating
Rattus norvegicus
-
2.3.1.15
additional information
distribution, overview
Escherichia coli
-
2.3.1.15
additional information
distribution, overview
Rattus norvegicus
-
2.3.1.20
adipocyte
-
Rattus norvegicus
-
2.3.1.20
intestinal mucosa
-
Rattus norvegicus
-
2.3.1.20
liver
-
Rattus norvegicus
-
2.3.1.20
mammary gland
-
Rattus norvegicus
-
2.3.1.20
additional information
distribution, overview
Rattus norvegicus
-
2.3.1.42
adipocyte
-
Rattus norvegicus
-
2.3.1.42
additional information
distribution, overview
Oryctolagus cuniculus
-
2.3.1.42
additional information
distribution, overview
Rattus norvegicus
-
2.3.1.51
liver
-
Rattus norvegicus
-
2.3.1.51
lung
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
saturated acyl-CoAs preferred
486338
Escherichia coli
CoA + 1-acyl-sn-glycerol 3-phosphate
-
-
-
?
2.3.1.15
acyl-CoA + sn-glycerol 3-phosphate
saturated acyl-CoAs preferred
486338
Rattus norvegicus
CoA + 1-acyl-sn-glycerol 3-phosphate
-
-
-
?
2.3.1.20
acyl-CoA + 1,2-diacylglycerol
last reaction in triacylglycerol synthesis
486338
Rattus norvegicus
CoA + triacylglycerol
-
-
-
?
2.3.1.42
acyl-CoA + dihydroxyacetone phosphate
-
486338
Rattus norvegicus
CoA + acyldihydroxyacetone phosphate
i.e. acylglycerone phosphate
486338
Rattus norvegicus
?
2.3.1.42
acyl-CoA + dihydroxyacetone phosphate
-
486338
Oryctolagus cuniculus
CoA + acyldihydroxyacetone phosphate
i.e. acylglycerone phosphate
486338
Oryctolagus cuniculus
?
2.3.1.42
palmitoyl-CoA + dihydroxyacetone phosphate
peroxisomal enzyme involved in ether lipid biosynthesis
486338
Rattus norvegicus
CoA + monopalmitoyldihydroxyacetone phosphate
-
-
-
?
2.3.1.42
palmitoyl-CoA + dihydroxyacetone phosphate
peroxisomal enzyme involved in ether lipid biosynthesis
486338
Oryctolagus cuniculus
CoA + monopalmitoyldihydroxyacetone phosphate
-
-
-
?
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
486338
Rattus norvegicus
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
i.e. phosphatidic acid
486338
Rattus norvegicus
?
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
involved in phospholipid biosynthesis
486338
Rattus norvegicus
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
486338
Rattus norvegicus
?
2.3.1.51
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
486338
Rattus norvegicus
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
-
486338
Rattus norvegicus
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.42
5.5
-
membrane-bound peroxisomal enzyme
Oryctolagus cuniculus
2.3.1.42
5.5
-
membrane-bound peroxisomal enzyme
Rattus norvegicus
2.3.1.42
7.5
-
microsomal enzyme
Oryctolagus cuniculus
2.3.1.42
7.5
-
microsomal enzyme
Rattus norvegicus