Literature summary extracted from

Kreme, L.; Maughan, W.N.; Wilson, R.A.; Dover, L.G.; Besra, G.S.
The M. tuberculosis antigen 85 complex and mycolyltransferase activity (2002), Lett. Appl. Microbiol., 34, 233-237.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.3.1.122	expression of FbpA, FbpB and FbpC2 in Escherichia coli, FbpB poorly expressed due to differential efficiency of mRNA translation	Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
2.3.1.122	-	Mycobacterium tuberculosis

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.3.1.122	6-azido-6-deoxytrehalose	inhibits all three members of Ag85 complex in vitro	Mycobacterium tuberculosis

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.3.1.122	30000	-	FbpA: SDS-PAGE, Western Blot, aminoacid sequence. FbpB: aminoacid sequence. FbpC2: SDS-PAGE, Western Blot, aminoacid sequence	Mycobacterium tuberculosis

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.3.1.122	Mycobacterium tuberculosis	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.3.1.122	recombinant proteins purified by affinity chromatography on Ni2+ charged His-trap columns	Mycobacterium tuberculosis

Reaction

EC Number	Reaction	Comment	Organism	Reaction ID
2.3.1.122	2 alpha,alpha-trehalose 6-mycolate = alpha,alpha-trehalose + alpha,alpha-trehalose 6,6'-bismycolate	reaction catalyzed by 3 members of the Ag85 complex in vitro, namely FbpA, FbpB and FbpC2	Mycobacterium tuberculosis	a

Subunits

EC Number	Subunits	Comment	Organism
2.3.1.122	monomer	FbpA: 1 * 30000, SDS-PAGE. FbpC2: 1 * 30000, SDS-PAGE	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)