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Literature summary extracted from

  • Hamada, M.; Otsuka, K.I.; Tanaka, N.; Ogasahara, K.; Koike, K.; Hiraoka, T.; Koike, M.
    Purification properties and subunit composition of pig heart lipoate acetyltransferase (1975), J. Biochem., 78, 187-197.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.12 freezing and thawing does not cause loss of activity Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.12 74000
-
 SDS-PAGE and sedimentation equilibrium data Sus scrofa
2.3.1.12 1800000 1970000 calculation from sedimentation and diffusion constants, meniscus depletion method Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.12 dihydrolipoamide + acetyl-CoA Sus scrofa
-
 S-acetyldihydrolipoamide + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.12 Sus scrofa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.12 lipoprotein 23 mol of lipoic acid per mol of enzyme Sus scrofa

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.12
-
 Sus scrofa

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.3.1.12 reconstitution of pyruvate dehydrogenase complex, optimum catalytic stoichiometry of E1:E2:E3 is 5.2:11.5:2 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.12 heart
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.12 19.7
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
2.3.1.12 -18°C, 0.05 mM potassium phosphate buffer, pH 7.0, 0.5 mM EDTA, more than 6 months Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.12 dihydrolipoamide + acetyl-CoA
-
 Sus scrofa S-acetyldihydrolipoamide + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.12 polymer 24 * 74000 SDS-PAGE, gel filtration in 6 M guanidine-HCl, sedimentation equilibrium in 6 M guanidine-HCl Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.12 25
-
-
 Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.12 60
-
 stable up to Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.12 7.8 8.5
-
 Sus scrofa