BRENDA - Enzyme Database

Purification and properties of a succinyltransferase from Pseudomonas aeruginosa specific for both arginine and ornithine

Tricot, C.; Vander Wauven, C.; Wattiez, R.; Falmagne, P.; Stalon, V.; Eur. J. Biochem. 224, 853-861 (1994)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.109
L-arginine
allosteric activator of the ornithine succinyltransferase activity
Pseudomonas aeruginosa
2.3.1.109
L-lysine
activator of the ornithine succinyltransferase activity
Pseudomonas aeruginosa
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.109
agmatine
-
Pseudomonas aeruginosa
2.3.1.109
D-arginine
competitive inhibition
Pseudomonas aeruginosa
2.3.1.109
spermidine
-
Pseudomonas aeruginosa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.109
0.04
-
L-ornithine
-
Pseudomonas aeruginosa
2.3.1.109
0.5
-
L-arginine
-
Pseudomonas aeruginosa
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.109
35000
-
2 * 35000 + 2 * 37000, heterotetramer, SDS-PAGE
Pseudomonas aeruginosa
2.3.1.109
37000
-
2 * 35000 + 2 * 37000, heterotetramer, SDS-PAGE
Pseudomonas aeruginosa
2.3.1.109
140000
-
PAGE
Pseudomonas aeruginosa
2.3.1.109
150000
-
gel filtration
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.109
succinyl-CoA + L-arginine
Pseudomonas aeruginosa
-
CoA + N2-succinyl-L-arginine
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.71
Pseudomonas aeruginosa
-
-
-
2.3.1.109
Pseudomonas aeruginosa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.109
-
Pseudomonas aeruginosa
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.109
-20C, retains full activity with both substrates for several weeks, loses 10-40% of its activity following repeated freezing and thawing
Pseudomonas aeruginosa
2.3.1.109
4C, 10 mM potassium phosphate, pH 7.5, stable for 48 h
Pseudomonas aeruginosa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.109
additional information
D-arginine, D-ornithine, L-lysine, L-citrulline, 2,4-aminobutyrate, 5-aminovalerate, 2-aminovalerate, agmatine or the polyamines propanediamine, putrescine, cadaverine and spermidine are not substrates
486122
Pseudomonas aeruginosa
?
-
-
-
-
2.3.1.109
succinyl-CoA + L-arginine
-
486122
Pseudomonas aeruginosa
CoA + N2-succinyl-L-arginine
-
-
-
?
2.3.1.109
succinyl-CoA + L-homoarginine
-
486122
Pseudomonas aeruginosa
CoA + N2-succinyl-L-homoarginine
-
-
-
?
2.3.1.109
succinyl-CoA + L-ornithine
-
486122
Pseudomonas aeruginosa
CoA + N2-succinyl-L-ornithine
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.3.1.109
tetramer
2 * 35000 + 2 * 37000, heterotetramer, SDS-PAGE
Pseudomonas aeruginosa
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.109
8.5
-
L-homoarginine as substrate
Pseudomonas aeruginosa
2.3.1.109
8.7
-
L-arginine as substrate
Pseudomonas aeruginosa
2.3.1.109
9
-
L-ornithine as substrate
Pseudomonas aeruginosa
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.3.1.109
0.9
-
spermidine
-
Pseudomonas aeruginosa
2.3.1.109
2.6
-
D-arginine
-
Pseudomonas aeruginosa
2.3.1.109
3.4
-
agmatine
-
Pseudomonas aeruginosa
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.109
L-arginine
allosteric activator of the ornithine succinyltransferase activity
Pseudomonas aeruginosa
2.3.1.109
L-lysine
activator of the ornithine succinyltransferase activity
Pseudomonas aeruginosa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.109
agmatine
-
Pseudomonas aeruginosa
2.3.1.109
D-arginine
competitive inhibition
Pseudomonas aeruginosa
2.3.1.109
spermidine
-
Pseudomonas aeruginosa
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.3.1.109
0.9
-
spermidine
-
Pseudomonas aeruginosa
2.3.1.109
2.6
-
D-arginine
-
Pseudomonas aeruginosa
2.3.1.109
3.4
-
agmatine
-
Pseudomonas aeruginosa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.109
0.04
-
L-ornithine
-
Pseudomonas aeruginosa
2.3.1.109
0.5
-
L-arginine
-
Pseudomonas aeruginosa
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.109
35000
-
2 * 35000 + 2 * 37000, heterotetramer, SDS-PAGE
Pseudomonas aeruginosa
2.3.1.109
37000
-
2 * 35000 + 2 * 37000, heterotetramer, SDS-PAGE
Pseudomonas aeruginosa
2.3.1.109
140000
-
PAGE
Pseudomonas aeruginosa
2.3.1.109
150000
-
gel filtration
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.109
succinyl-CoA + L-arginine
Pseudomonas aeruginosa
-
CoA + N2-succinyl-L-arginine
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.109
-
Pseudomonas aeruginosa
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.109
-20C, retains full activity with both substrates for several weeks, loses 10-40% of its activity following repeated freezing and thawing
Pseudomonas aeruginosa
2.3.1.109
4C, 10 mM potassium phosphate, pH 7.5, stable for 48 h
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.109
additional information
D-arginine, D-ornithine, L-lysine, L-citrulline, 2,4-aminobutyrate, 5-aminovalerate, 2-aminovalerate, agmatine or the polyamines propanediamine, putrescine, cadaverine and spermidine are not substrates
486122
Pseudomonas aeruginosa
?
-
-
-
-
2.3.1.109
succinyl-CoA + L-arginine
-
486122
Pseudomonas aeruginosa
CoA + N2-succinyl-L-arginine
-
-
-
?
2.3.1.109
succinyl-CoA + L-homoarginine
-
486122
Pseudomonas aeruginosa
CoA + N2-succinyl-L-homoarginine
-
-
-
?
2.3.1.109
succinyl-CoA + L-ornithine
-
486122
Pseudomonas aeruginosa
CoA + N2-succinyl-L-ornithine
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.3.1.109
tetramer
2 * 35000 + 2 * 37000, heterotetramer, SDS-PAGE
Pseudomonas aeruginosa
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.109
8.5
-
L-homoarginine as substrate
Pseudomonas aeruginosa
2.3.1.109
8.7
-
L-arginine as substrate
Pseudomonas aeruginosa
2.3.1.109
9
-
L-ornithine as substrate
Pseudomonas aeruginosa