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Literature summary extracted from

  • Dahlbender, B.; Strack, D.
    Purification and properties of 1-(hydroxycinnamoyl)-glucose:1-(hydroxycinnamoyl)-glucose hydroxycinnamoyl-transferase from radish seedlings (1986), Phytochemistry, 25, 1043-1046.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.103 additional information no requirement for divalent cations or thiols Raphanus sativus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.103 2-mercaptoethanol 1 mM, total inhibition Raphanus sativus
2.3.1.103 Ca2+ divalent cations at concentrations of more than 5 mM are inhibitory, 10 mM, 45% remaining activity Raphanus sativus
2.3.1.103 Co2+ divalent cations at concentrations of more than 5 mM are inhibitory, 10 mM, total inhibition Raphanus sativus
2.3.1.103 dithiothreitol 1 mM, total inhibition Raphanus sativus
2.3.1.103 Mg2+ divalent cations at concentrations of more than 5 mM are inhibitory, 10 mM, 65% remaining activity Raphanus sativus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.103 0.4
-
 1-feruloyl-beta-D-glucose
-
 Raphanus sativus
2.3.1.103 0.42
-
 1-sinapoyl-beta-D-glucose
-
 Raphanus sativus
2.3.1.103 0.58
-
 1-(p-coumaroyl)-glucose
-
 Raphanus sativus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.103 55000
-
 gel filtration, SDS-PAGE Raphanus sativus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.103 1-O-sinapoyl-beta-D-glucopyranose + 1-O-sinapoyl-beta-D-glucopyranose Raphanus sativus
-
 1,2-di-O-sinapoyl-beta-D-glucopyranose + glucose
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.103 Raphanus sativus
-
 L. var. sativus cv. Saxa
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.103 partial Raphanus sativus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.103 cotyledon dark grown seedlings show higher activity than light grown seedlings Raphanus sativus
-

Storage Stability

EC Number Storage Stability Organism
2.3.1.103 -20°C, presence of 3 mg/ml bovine serum albumin, at least 1 year Raphanus sativus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.103 1-(p-coumaroyl)-beta-D-glucose + 1-(p-coumaroyl)-beta-D-glucose
-
 Raphanus sativus 1,2-di-O-p-coumaroyl-beta-D-glucopyranose + D-glucose
-
 ir
2.3.1.103 1-feruloyl-beta-D-glucose + 1-feruloyl-beta-D-glucose
-
 Raphanus sativus 1,2-di-O-feruloyl-beta-glucopyranose + glucose
-
 ir
2.3.1.103 1-O-sinapoyl-beta-D-glucopyranose + 1-O-sinapoyl-beta-D-glucopyranose
-
 Raphanus sativus 1,2-di-O-sinapoyl-beta-D-glucopyranose + glucose
-
 ir
2.3.1.103 additional information strict specificity of acyl transfer to the C2 hydroxyl group of the acceptor glucose molecule Raphanus sativus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.103 monomer 1 * 55000, SDS-PAGE Raphanus sativus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.103 42
-
-
 Raphanus sativus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.103 8
-
 HEPES-buffer Raphanus sativus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.103 7 9 50% of maximal activity at pH 7.0 and pH 9.0 Raphanus sativus