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Literature summary extracted from

  • Murphy, D.J.; Walker, D.A.
    The properties from transketolase from photosynthetic tissue (1982), Planta, 155, 316-320.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.2.1.1 chloroplast no stimulation by light Triticum aestivum 9507
-
2.2.1.1 chloroplast no stimulation by light Spinacia oleracea 9507
-
2.2.1.1 cytosol
-
 Triticum aestivum 5829
-
2.2.1.1 cytosol
-
 Spinacia oleracea 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.1 Mg2+ requirement Triticum aestivum
2.2.1.1 Mg2+ requirement Spinacia oleracea
2.2.1.1 Mg2+ chloroplastic, not cytosolic enzyme Triticum aestivum
2.2.1.1 Mg2+ chloroplastic, not cytosolic enzyme Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.1 37600
-
 4 * 37600, holoenzyme, SDS-PAGE Triticum aestivum
2.2.1.1 150000
-
 gel filtration Triticum aestivum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate Triticum aestivum
-
 sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate Spinacia oleracea
-
 sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.1 Spinacia oleracea
-
 spinach
-
2.2.1.1 Triticum aestivum
-
 wheat, cv. Sappo
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.1
-
 Triticum aestivum
2.2.1.1
-
 Spinacia oleracea

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.2.1.1 thiamine diphosphate reconstitutes dissociated holoenzyme Triticum aestivum
2.2.1.1 thiamine diphosphate reconstitutes dissociated holoenzyme Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.2.1.1 leaf
-
 Triticum aestivum
-
2.2.1.1 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2.1.1 1.6
-
-
 Triticum aestivum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.1 D-xylulose 5-phosphate + D-erythrose 4-phosphate
-
 Triticum aestivum D-fructose 6-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-erythrose 4-phosphate
-
 Spinacia oleracea D-fructose 6-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate
-
 Triticum aestivum sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate
-
 Spinacia oleracea sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate D-erythrose 4-phosphate Triticum aestivum sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate D-erythrose 4-phosphate Spinacia oleracea sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate sedoheptulose 7-phosphate Triticum aestivum sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate sedoheptulose 7-phosphate Spinacia oleracea sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate wide specificity for both reactants: donor substrates may be: fructose 6-phosphate Triticum aestivum sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate wide specificity for both reactants: donor substrates may be: fructose 6-phosphate Spinacia oleracea sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.2.1.1 dimer
-
 Spinacia oleracea
2.2.1.1 dimer apoenzyme, gel filtration after 2-mercaptoethanol incubation Triticum aestivum
2.2.1.1 More catalytically active apoenzyme dimer and holoenzyme tetramer Triticum aestivum
2.2.1.1 More tetramer dissociates into dimers in the absence of thiamine diphosphate or in dilute solution Triticum aestivum
2.2.1.1 tetramer 4 * 37600, holoenzyme, SDS-PAGE Triticum aestivum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.1 7.5 8.5
-
 Triticum aestivum
2.2.1.1 7.5 8.5
-
 Spinacia oleracea
2.2.1.1 8.5 9
-
 Triticum aestivum
2.2.1.1 8.5 9
-
 Spinacia oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.1 thiamine diphosphate
-
 Triticum aestivum
2.2.1.1 thiamine diphosphate
-
 Spinacia oleracea