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Literature summary extracted from

  • Coque, J.J.R.; Perez-Llarena, F.J.; Enguita, F.J.; Fuente, J.L.; Martin, J.F.; Liras, P.
    Characterization of the cmcH genes of Nocardia lactamdurans and Streptomyces clavuligerus encoding a functional 3'-hydroxymethylcephem O-carbamoyltransferase for cephamycin biosynthesis (1995), Gene, 162, 21-27.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.3.3
-
 Amycolatopsis lactamdurans
2.1.3.3
-
 Streptomyces clavuligerus
2.1.3.7 cmcH subcloned in pIJ702 and expressed in Streptomyces lividans Amycolatopsis lactamdurans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.3 57148
-
 x * 57148, deduced from nucleotide sequence Amycolatopsis lactamdurans
2.1.3.7 57149
-
 x * 57149, calculation from nucleotide sequence Amycolatopsis lactamdurans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.3.7 carbamoyl phosphate + a 3-hydroxymethyl ceph-3-em-4-carboxylate Amycolatopsis lactamdurans enzyme is involved in biosynthesis of cephamycin phosphate + a 3-carbamoyloxymethylcephem
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.3 Amycolatopsis lactamdurans
-
 3'-hydroxymethylcephem O-carbamoyltransferase
-
2.1.3.3 Streptomyces clavuligerus
-
 3'-hydroxymethylcephem O-carbamoyltransferase
-
2.1.3.7 Amycolatopsis lactamdurans
-
-
-
2.1.3.7 no activity in Streptomyces griseus
-
-
-
2.1.3.7 no activity in Streptomyces lipmanii
-
-
-
2.1.3.7 Streptomyces cattleya
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.3 3'-hydroxymethylcephem + carbamoyl phosphate
-
 Amycolatopsis lactamdurans cephamycin C + phosphate
-
 ?
2.1.3.3 3'-hydroxymethylcephem + carbamoyl phosphate
-
 Streptomyces clavuligerus cephamycin C + phosphate
-
 ?
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Amycolatopsis lactamdurans phosphate + L-citrulline
-
 ?
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Streptomyces clavuligerus phosphate + L-citrulline
-
 ?
2.1.3.7 carbamoyl phosphate + a 3-hydroxymethyl ceph-3-em-4-carboxylate enzyme is involved in biosynthesis of cephamycin Amycolatopsis lactamdurans phosphate + a 3-carbamoyloxymethylcephem
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.3.3 ? x * 57148, deduced from nucleotide sequence Amycolatopsis lactamdurans
2.1.3.7 ? x * 57149, calculation from nucleotide sequence Amycolatopsis lactamdurans