EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.3.3 | expression of wild-type, Y227C, A240D, E277G, Y227C/E277G and A240D/E277G mutant enzyme in Saccharomyces cerevisiae | Pyrococcus furiosus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.3.3 | A240D | 10fold increase of Km at 55°C | Pyrococcus furiosus |
2.1.3.3 | E277G | 14fold increase of Km at 55°C | Pyrococcus furiosus |
2.1.3.3 | Y227D | slight increase of Km at 55°C | Pyrococcus furiosus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.3.3 | Pyrococcus furiosus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.3.3 | recombinant enzyme, Mono Q, arginine-Sepharose column | Pyrococcus furiosus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.3.3 | carbamoyl phosphate + L-ornithine | - |
Pyrococcus furiosus | phosphate + L-citrulline | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.3.3 | 370 | - |
ornithine | at 30°C | Pyrococcus furiosus | |
2.1.3.3 | 500 | - |
ornithine | at 55°C | Pyrococcus furiosus | |
2.1.3.3 | 2200 | - |
ornithine | Y227C/E277G double mutant, at 30°C | Pyrococcus furiosus | |
2.1.3.3 | 2900 | - |
ornithine | A240D/E277G double mutant, at 30°C | Pyrococcus furiosus | |
2.1.3.3 | 3500 | - |
ornithine | Y227C/E277G double mutant, at 55°C | Pyrococcus furiosus | |
2.1.3.3 | 4300 | - |
ornithine | A240D/E277G double mutant, at 55°C | Pyrococcus furiosus |