EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.1.3.3 | additional information | activity increases with ionic strength up to 100 mM | Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.3.3 | coexpression with GroES and GroEL in Escherichia coli | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.3.3 | R277W | shows no substrate inhibition by ornithine, 70fold lower affinity for L-ornithine | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.3.3 | 2-mercaptoethanol | - |
Homo sapiens | |
2.1.3.3 | dithiothreitol | - |
Homo sapiens | |
2.1.3.3 | ornithine | recombinant wild-type enzyme | Homo sapiens | |
2.1.3.3 | Zn2+ | - |
Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.3.3 | Homo sapiens | - |
ornithine transcarbamoyltransferase deficiency is the most common cause of inherited urea cycle disorders | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.3.3 | recombinant wild-type and R277W mutant enzyme, ammonium sulfate, delta-N-(phosphonacetyl)-L-ornithine affinity chromatography | Homo sapiens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.1.3.3 | 40.2 | - |
recombinant enzyme | Homo sapiens |
EC Number | Storage Stability | Organism |
---|---|---|
2.1.3.3 | -20°C, 20 mM Tris acetate, 2 mM EDTA, pH 7.4, 50% glycerol, no loss of activity | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.3.3 | carbamoyl phosphate + L-ornithine | - |
Homo sapiens | phosphate + L-citrulline | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.3.3 | 51 | - |
R277W mutant enzyme, 50% loss of activity | Homo sapiens |
2.1.3.3 | 56 | - |
recombinant enzyme, 50% loss of activity | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.3.3 | 8 | - |
gradual loss of activity above pH 8.5 or below pH 6.5 | Homo sapiens |