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Literature summary extracted from

  • Vickrey, J.F.; Herve, G.; Evans, D.R.
    Pseudomonas aeruginosa aspartate transcarbamoylase. Characterization of its catalytic and regulatory properties (2002), J. Biol. Chem., 277, 24490-24498.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.3.2 N-(Phosphonoacetyl)-L-aspartate at concentrations below 0.000002 mM, inhibition above Pseudomonas aeruginosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.3.2 expression in Escherichia coli Pseudomonas aeruginosa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.3.2 hanging drop method, equal volumes of 1 mg/ml enzyme in 100 mM Tris-HCl, pH 8.5 is mixed with 100 mM citrate, pH 5, and 10% 6K polyethylene glycol Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.3.2 ATP excess MgCl2 abolishes inhibition Pseudomonas aeruginosa
2.1.3.2 CTP
-
 Pseudomonas aeruginosa
2.1.3.2 GTP
-
 Pseudomonas aeruginosa
2.1.3.2 UTP
-
 Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.3.2 0.49
-
 Carbamoyl phosphate
-
 Pseudomonas aeruginosa
2.1.3.2 2.6
-
 L-aspartate
-
 Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.2 36000
-
 6 * 45000 + 6 * 36000, SDS-PAGE Pseudomonas aeruginosa
2.1.3.2 45000
-
 6 * 45000 + 6 * 36000, SDS-PAGE Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.3.2 carbamoylphosphate + L-aspartate Pseudomonas aeruginosa
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.2 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.2 recombinant enzyme, Ni2+-affinity column Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.3.2 450
-
 recombinant enzyme Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Pseudomonas aeruginosa phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Pseudomonas aeruginosa phosphate + N-carbamoyl-L-aspartate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.3.2 dodecamer 6 * 45000 + 6 * 36000, SDS-PAGE Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.3.2 8.5
-
-
 Pseudomonas aeruginosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.3.2 7 8.5 steep increase in activity until pH 8.5, decrase above Pseudomonas aeruginosa