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Literature summary extracted from

  • Ong, B.L.; Jackson, J.F.
    Aspartate transcarbmoylase from Phaseolus aureus (1972), Biochem. J., 129, 571-581.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.1.3.2 phosphate required for maximum stability Vigna radiata var. radiata
2.1.3.2 presence of 20 mM potassium phosphate as well as 10% v/v glycerol is required for maintenance of activity of the purified enzyme Vigna radiata var. radiata

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.3.2 carbamoyl aspartate
-
 Vigna radiata var. radiata
2.1.3.2 Guanidine-HCl 800 mM, almost complete inhibition Vigna radiata var. radiata
2.1.3.2 p-chloromercuribenzoate 6 mM, almost complete inhibition Vigna radiata var. radiata
2.1.3.2 phosphate
-
 Vigna radiata var. radiata
2.1.3.2 succinate activator at low concentrations of both succinate and aspartate, inhibitor at high succinate concentrations and at high aspartate concentrations Vigna radiata var. radiata
2.1.3.2 UMP
-
 Vigna radiata var. radiata
2.1.3.2 Urea 2.5 M, almost complete inhibition Vigna radiata var. radiata
2.1.3.2 UTP
-
 Vigna radiata var. radiata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.3.2 0.091
-
 Carbamoyl phosphate
-
 Vigna radiata var. radiata
2.1.3.2 1.7
-
 L-aspartate
-
 Vigna radiata var. radiata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.2 83000
-
 gel filtration Vigna radiata var. radiata
2.1.3.2 93000
-
 glycerol gradient Vigna radiata var. radiata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.3.2 carbamoylphosphate + L-aspartate Vigna radiata var. radiata
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.2 Vigna radiata var. radiata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.2 ammonium sulfate, DEAE-cellulose, DEAE-Sephadex, Sephadex G-200, partially purified Vigna radiata var. radiata

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.3.2 carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Vigna radiata var. radiata
a
2.1.3.2 carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate kinetic data suggest an ordered bi bi mechanism Vigna radiata var. radiata
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.3.2 0.35
-
-
 Vigna radiata var. radiata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Vigna radiata var. radiata phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Vigna radiata var. radiata phosphate + N-carbamoyl-L-aspartate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.3.2 10.5
-
-
 Vigna radiata var. radiata