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Literature summary extracted from

  • Newton, D.T.; Mangroo, D.
    Mapping the active site of the Haemophilus influenzae methionyl-tRNA formyltransferase: Residues important for catalysis and tRNA binding (1999), Biochem. J., 339, 63-69.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.9 cloning of the fmt gene and expression of wild-type and mutant genes in Escherichia coli BL21(DE3) as His6-tagged proteins Haemophilus influenzae
2.1.2.9 fmt gene encoding enzyme is cloned Thermus thermophilus
2.1.2.9 fmt gene encoding enzyme is cloned Pseudomonas aeruginosa
2.1.2.9 fmt gene encoding enzyme is cloned Rickettsia prowazekii

Protein Variants

EC Number Protein Variants Comment Organism
2.1.2.9 D145A mutant enzyme with drastically reduced activity Haemophilus influenzae
2.1.2.9 D145E mutant enzyme with 881fold reduced formylation rate, kinetic parameters Haemophilus influenzae
2.1.2.9 D145N mutant enzyme with 4031fold reduced formylation rate, kinetic parameters Haemophilus influenzae
2.1.2.9 H109A mutant enzyme with 4677fold reduced formylation rate, kinetic parameters Haemophilus influenzae
2.1.2.9 H109K inactive mutant enzyme Haemophilus influenzae
2.1.2.9 H109R mutant enzyme with 7172fold reduced formylation rate, kinetic parameters Haemophilus influenzae
2.1.2.9 K37A mutant enzyme with 3.5fold reduced formylation rate, kinetic parameters Haemophilus influenzae
2.1.2.9 additional information mutations of Asp-145 and Arg-41 reduce the affinity for initiator tRNA, mutations of Asn-107 and His-109 affect catalysis but not tRNA binding Haemophilus influenzae
2.1.2.9 N107A mutant enzyme with 48fold reduced formylation rate, kinetic parameters Haemophilus influenzae
2.1.2.9 R41A mutant enzyme with 470fold reduced formylation rate, kinetic parameters Haemophilus influenzae
2.1.2.9 R41K mutant enzyme with 140fold reduced formylation rate, kinetic parameters Haemophilus influenzae
2.1.2.9 V31A mutant enzyme with 1.7fold reduced formylation rate Haemophilus influenzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.9 additional information
-
 additional information values for several mutant enzymes Haemophilus influenzae
2.1.2.9 0.00112
-
 E.coli L-methionyl-tRNAfMet
-
 Haemophilus influenzae

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.9 Haemophilus influenzae
-
-
-
2.1.2.9 Pseudomonas aeruginosa
-
-
-
2.1.2.9 Rickettsia prowazekii
-
-
-
2.1.2.9 Thermus thermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.9 purification of His6-tagged wild-type and mutant recombinant enzymes, expressed in Escherichia coli BL21(DE3) Haemophilus influenzae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.9 additional information
-
-
 Haemophilus influenzae
2.1.2.9 1.23
-
-
 Haemophilus influenzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.9 10-formyltetrahydrofolate + E. coli L-methionyl-tRNAfMet
-
 Haemophilus influenzae tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 r
2.1.2.9 10-formyltetrahydrofolate + E.coli L-methionyl-tRNAfMet
-
 Haemophilus influenzae tetrahydrofolate + E.coli N-formylmethionyl-tRNAfMet
-
 r
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O formylates methionyl-tRNAfMet from E. coli Haemophilus influenzae tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Asn-107, His-109 and Asp-145 are catalytic residues, Arg-41 is involved in tRNA recognition Haemophilus influenzae tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.2.9 37
-
 assay at Haemophilus influenzae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.2.9 additional information
-
 additional information values for several mutant enzymes Haemophilus influenzae
2.1.2.9 11.3
-
 E. coli L-methionyl-tRNAfMet
-
 Haemophilus influenzae