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Literature summary extracted from

  • Bulock, K.G.; Beardsley, G.P.; Anderson, K.S.
    The kinetic mechanism of the human bifunctional enzyme ATIC (5-amino-4-imidazolecarboxamide ribonucleotide transformylase/inosine 5'-monophosphate cyclohydrolase): A surprising lack of substrate channeling (2002), J. Biol. Chem., 277, 22168-22174.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.2.3 medicine
-
 Gallus sp.
2.1.2.3 medicine target for development of inhibitors with potential use as chemotherapeutic agents Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.2.3 ATIC complex crystallized in monoclinic space group P2(1), a = 56.48 A, b = 107.88 A, c = 103.86 A and beta = 91.2° Gallus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.3 (6S/R)-5,10-dideaza-5,6,7,8,-tetrahydrofolate
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.3 0.01
-
 10-formyltetrahydrofolate
-
 Homo sapiens
2.1.2.3 0.185
-
 tetrahydrofolate
-
 Homo sapiens
3.5.4.10 0.0014
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.4 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide Homo sapiens penultimate and final steps in the de novo purine biosynthesis pathway tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
-
 r
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide Gallus sp. penultimate and final steps in the de novo purine biosynthesis pathway tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
-
 r
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide Homo sapiens reduced folate cofactor requiring step of de novo purine biosynthesis tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
-
 r
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide Gallus sp. reduced folate cofactor requiring step of de novo purine biosynthesis tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.3 Gallus sp.
-
 avian
-
2.1.2.3 Homo sapiens
-
 human
-
3.5.4.10 Homo sapiens
-
 bifunctional enzyme 5-amino-4-imidazolecarboxamide ribonucleotide transformylase/inosine 5'-monophosphate cyclohydrolase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.3
-
 Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.4.10 IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide mechanism,kinetics, enzyme reaction draws the overall bifunctional reaction towards the IMP product Homo sapiens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide penultimate and final steps in the de novo purine biosynthesis pathway Homo sapiens tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
-
 r
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide penultimate and final steps in the de novo purine biosynthesis pathway Gallus sp. tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
-
 r
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide reduced folate cofactor requiring step of de novo purine biosynthesis Homo sapiens tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
-
 r
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide reduced folate cofactor requiring step of de novo purine biosynthesis Gallus sp. tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
-
 r
2.1.2.3 additional information in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene Homo sapiens ?
-
 ?
2.1.2.3 additional information in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene Gallus sp. ?
-
 ?
2.1.2.3 N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
-
 Homo sapiens tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
-
 r
2.1.2.3 N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
-
 Gallus sp. tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
-
 r
3.5.4.10 5-formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide
-
 Homo sapiens IMP + H2O
-
 ir

Synonyms

EC Number Synonyms Comment Organism
2.1.2.3 AICARFT
-
 Homo sapiens
2.1.2.3 ATIC
-
 Homo sapiens
2.1.2.3 ATIC
-
 Gallus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.3 N10-formyltetrahydrofolate
-
 Homo sapiens
2.1.2.3 N10-formyltetrahydrofolate
-
 Gallus sp.