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Literature summary extracted from

  • Kurtov, D.; Kinghorn, J.R.; Unkles, S.E.
    The Aspergillus nidulans panB gene encodes ketopantoate hydroxymethyltransferase, required for biosynthesis of pantothenate and coenzyme A (1999), Mol. Gen. Genet., 262, 115-120.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.2.11 pharmacology enzyme could serve as target for anti-fungal drugs, since it is not present in mammals Aspergillus nidulans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.11 panB gene encoding enzyme is cloned, sequenced and expressed in Escherichia coli as an active octameric enzyme, ORF encodes a protein of 349 amino acids, panB gene is closely linked to nimO gene on Aspergillus nidulans linkage group VII Aspergillus nidulans

Protein Variants

EC Number Protein Variants Comment Organism
2.1.2.11 additional information panB100 mutant has a deletion of G-268 Aspergillus nidulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.11 37700
-
8 * 37700, calculated from the amino acid sequence, 8 * 40000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE Aspergillus nidulans
2.1.2.11 40000
-
8 * 37700, calculated from the amino acid sequence, 8 * 40000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE Aspergillus nidulans
2.1.2.11 340000
-
recombinant enzyme expressed in Escherichia coli, gel filtration Aspergillus nidulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.11 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate Aspergillus nidulans essential for the biosynthesis of coenzyme A tetrahydrofolate + 2-dehydropantoate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.11 Aspergillus nidulans Q9Y7B6 wild-type strains biA1 and yA2, strain G1765
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.11 affinity purification of recombinant PanB Aspergillus nidulans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.11 0.01 0.027 recombinant enzyme expressed in Escherichia coli Aspergillus nidulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.11 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate
-
Aspergillus nidulans tetrahydrofolate + 2-dehydropantoate
-
?
2.1.2.11 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate essential for the biosynthesis of coenzyme A Aspergillus nidulans tetrahydrofolate + 2-dehydropantoate
-
?

Subunits

EC Number Subunits Comment Organism
2.1.2.11 octamer 8 * 37700, calculated from the amino acid sequence, 8 * 40000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE Aspergillus nidulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.2.11 37
-
assay at Aspergillus nidulans

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.11 5,10-methylenetetrahydrofolate
-
Aspergillus nidulans
2.1.2.11 tetrahydrofolate
-
Aspergillus nidulans