Literature summary extracted from
Kurtov, D.; Kinghorn, J.R.; Unkles, S.E.
The Aspergillus nidulans panB gene encodes ketopantoate hydroxymethyltransferase, required for biosynthesis of pantothenate and coenzyme A (1999), Mol. Gen. Genet., 262, 115-120.
Application
EC Number |
Application |
Comment |
Organism |
---|
2.1.2.11 |
pharmacology |
enzyme could serve as target for anti-fungal drugs, since it is not present in mammals |
Aspergillus nidulans |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.1.2.11 |
panB gene encoding enzyme is cloned, sequenced and expressed in Escherichia coli as an active octameric enzyme, ORF encodes a protein of 349 amino acids, panB gene is closely linked to nimO gene on Aspergillus nidulans linkage group VII |
Aspergillus nidulans |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.1.2.11 |
additional information |
panB100 mutant has a deletion of G-268 |
Aspergillus nidulans |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
2.1.2.11 |
37700 |
- |
8 * 37700, calculated from the amino acid sequence, 8 * 40000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE |
Aspergillus nidulans |
2.1.2.11 |
40000 |
- |
8 * 37700, calculated from the amino acid sequence, 8 * 40000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE |
Aspergillus nidulans |
2.1.2.11 |
340000 |
- |
recombinant enzyme expressed in Escherichia coli, gel filtration |
Aspergillus nidulans |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
2.1.2.11 |
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate |
Aspergillus nidulans |
essential for the biosynthesis of coenzyme A |
tetrahydrofolate + 2-dehydropantoate |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.1.2.11 |
Aspergillus nidulans |
Q9Y7B6 |
wild-type strains biA1 and yA2, strain G1765 |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.1.2.11 |
affinity purification of recombinant PanB |
Aspergillus nidulans |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
2.1.2.11 |
0.01 |
0.027 |
recombinant enzyme expressed in Escherichia coli |
Aspergillus nidulans |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.1.2.11 |
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate |
- |
Aspergillus nidulans |
tetrahydrofolate + 2-dehydropantoate |
- |
? |
|
2.1.2.11 |
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate |
essential for the biosynthesis of coenzyme A |
Aspergillus nidulans |
tetrahydrofolate + 2-dehydropantoate |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.1.2.11 |
octamer |
8 * 37700, calculated from the amino acid sequence, 8 * 40000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE |
Aspergillus nidulans |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
2.1.2.11 |
37 |
- |
assay at |
Aspergillus nidulans |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
2.1.2.11 |
5,10-methylenetetrahydrofolate |
- |
Aspergillus nidulans |
|
2.1.2.11 |
tetrahydrofolate |
- |
Aspergillus nidulans |
|