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Literature summary extracted from

  • Hauffe, K.D.; Hahlbrock, K.; Scheel, D.
    Elicitor-stimulated furanocoumarin biosynthesis in cultured parsley cells: S-adenosyl-L-methionine:bergaptol and S-adenosyl-L-methionine:xanthotoxol O-methyltransferase (1986), Z. Naturforsch. C, 41, 228-239.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.1.1.69 2-mercaptoethanol stabilizes during purification and storage Petroselinum crispum
2.1.1.70 2-mercaptoethanol stabilizes during purification and storage Petroselinum crispum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.70 phosphate
-
 Petroselinum crispum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.69 0.001
-
 5-hydroxyxanthotoxin
-
 Petroselinum crispum
2.1.1.69 0.0031
-
 S-adenosyl-L-methionine reaction with bergaptol or 5-hydroxyxanthotoxin Petroselinum crispum
2.1.1.69 0.004
-
 bergaptol
-
 Petroselinum crispum
2.1.1.70 0.0044
-
 S-adenosyl-L-methionine
-
 Petroselinum crispum
2.1.1.70 0.0098
-
 xanthotoxol
-
 Petroselinum crispum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.69 36000
-
 2 * 36000, SDS-PAGE Petroselinum crispum
2.1.1.69 73000
-
 gel filtration Petroselinum crispum
2.1.1.70 34000
-
 2 * 34000, SDS-PAGE Petroselinum crispum
2.1.1.70 67000
-
 gel filtration Petroselinum crispum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.69 S-adenosyl-L-methionine + bergaptol Petroselinum crispum final methylation in the biosynthesis of the furanocoumarin bergapten ?
-
 ?
2.1.1.70 S-adenosyl-L-methionine + xanthotoxol Petroselinum crispum final methylation in the biosynthesis of the furanocoumarin xanthotoxin S-adenosyl-L-homocysteine + xanthotoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.69 Petroselinum crispum
-
-
-
2.1.1.70 Petroselinum crispum
-
 cv Hamburger Schnitt
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.69
-
 Petroselinum crispum
2.1.1.70
-
 Petroselinum crispum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.69 cell suspension culture from leaf petiols, cells activated by an elicitor from Phytophtora megasperma, no activity in untreated cells Petroselinum crispum
-
2.1.1.70 cell suspension culture from leaf petiole, cells treated with an elicitor from Phytophtora megasperma, no activity in untreated cells Petroselinum crispum
-
2.1.1.70 petiole cell suspension culture Petroselinum crispum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.69 0.041
-
-
 Petroselinum crispum
2.1.1.70 0.155
-
-
 Petroselinum crispum

Storage Stability

EC Number Storage Stability Organism
2.1.1.69 -20 C, stable for 20 days in 50% v/v glycerol Petroselinum crispum
2.1.1.70 -20°C, stable for 20 days in 50% v/v glycerol Petroselinum crispum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.69 S-adenosyl-L-methionine + 5,7-dihydroxycoumarin 6% of the activity with bergaptol Petroselinum crispum S-adenosyl-L-homocysteine + citropten
-
 ?
2.1.1.69 S-adenosyl-L-methionine + 5,8-dihydroxypsoralen 2.6% of the activity with bergaptol Petroselinum crispum S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.69 S-adenosyl-L-methionine + 5-hydroxyxanthotoxin best substrate Petroselinum crispum S-adenosyl-L-homocysteine + isopimpinellin
-
 ?
2.1.1.69 S-adenosyl-L-methionine + bergaptol
-
 Petroselinum crispum S-adenosyl-L-homocysteine + bergapten
-
 ?
2.1.1.69 S-adenosyl-L-methionine + bergaptol final methylation in the biosynthesis of the furanocoumarin bergapten Petroselinum crispum ?
-
 ?
2.1.1.70 S-adenosyl-L-methionine + 5-hydroxyxanthotoxin 0.7% of the activity with xanthotoxol Petroselinum crispum S-adenosyl-L-homocysteine + isopimpinellin
-
 ?
2.1.1.70 S-adenosyl-L-methionine + 8-hydroxybergapten 2% of the activity with xanthotoxol Petroselinum crispum S-adenosyl-L-homocysteine + isopimpinellin
-
 ?
2.1.1.70 S-adenosyl-L-methionine + bergaptol 0.5% of the activity with xanthotoxol Petroselinum crispum S-adenosyl-L-homocysteine + bergapten
-
 ?
2.1.1.70 S-adenosyl-L-methionine + daphnetin 0.6% of the activity with xanthotoxol Petroselinum crispum S-adenosyl-L-homocysteine + hydrangetin
-
 ?
2.1.1.70 S-adenosyl-L-methionine + xanthotoxol
-
 Petroselinum crispum S-adenosyl-L-homocysteine + xanthotoxin
-
 ?
2.1.1.70 S-adenosyl-L-methionine + xanthotoxol final methylation in the biosynthesis of the furanocoumarin xanthotoxin Petroselinum crispum S-adenosyl-L-homocysteine + xanthotoxin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.69 dimer 2 * 36000, SDS-PAGE Petroselinum crispum
2.1.1.70 dimer 2 * 34000, SDS-PAGE Petroselinum crispum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.70 7.5 8
-
 Petroselinum crispum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.69 8 8.5
-
 Petroselinum crispum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.69 6.5 9.3 pH 6.5: about 30% of maximal activity, pH 9.3: about 45% of maximal activity Petroselinum crispum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.1.1.69 5
-
 below: inactivation within 30 min Petroselinum crispum
2.1.1.70 5
-
 30 min, inactivation below Petroselinum crispum