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Literature summary extracted from

  • Vijayasarathy, C.; Rao, B.S.N.
    Partial purification and characterisation of S-adenosylmethionine:protein-histidine N-methyltransferase from rabbit skeletal muscle (1987), Biochim. Biophys. Acta, 923, 156-165.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.85 additional information reducing agents e.g. mercaptoethanol, required for activity, 4fold stimulation Oryctolagus cuniculus
2.1.1.85 Triton X-100 stimulation at 1% Oryctolagus cuniculus

General Stability

EC Number General Stability Organism
2.1.1.85 2-mercaptoethanol or glycerol does not stabilize Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.85 ATP 47% inhibition at 0.4 mM Oryctolagus cuniculus
2.1.1.85 Borate slight inhibition Oryctolagus cuniculus
2.1.1.85 Ca2+ 30% inhibition at 0.4 mM Oryctolagus cuniculus
2.1.1.85 EDTA slight inhibition Oryctolagus cuniculus
2.1.1.85 K+ 72% inhibition at 200 mM Oryctolagus cuniculus
2.1.1.85 Mg2+ 60% inhibition at 8 mM Oryctolagus cuniculus
2.1.1.85 N-ethylmaleimide 70% inhibition at 4 mM Oryctolagus cuniculus
2.1.1.85 Na+ 95% inhibition at 200 mM Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.85 myofibril
-
 Oryctolagus cuniculus 30016
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.85 additional information no requirement for any metal ion Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.85 S-adenosyl-L-methionine + protein L-histidine Oryctolagus cuniculus
-
 S-adenosyl-L-homocysteine + protein Ntau-methyl-L-histidine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.85 Oryctolagus cuniculus
-
 rabbit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.85 partial Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.85 skeletal muscle
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.85 additional information
-
 different fractions after ammonium sulfate precipitation Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
2.1.1.85 -20°C Oryctolagus cuniculus
2.1.1.85 2°C, 4 days, 75% loss of activity, no stabilization by mercaptoethanol or glycerol Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.85 S-adenosyl-L-methionine + protein L-histidine
-
 Oryctolagus cuniculus S-adenosyl-L-homocysteine + protein Ntau-methyl-L-histidine
-
 ?
2.1.1.85 S-adenosyl-L-methionine + protein L-histidine specifically methylates histidine residues of actin Oryctolagus cuniculus S-adenosyl-L-homocysteine + protein Ntau-methyl-L-histidine
-
 ?
2.1.1.85 S-adenosyl-L-methionine + protein L-histidine some activity with gluten Oryctolagus cuniculus S-adenosyl-L-homocysteine + protein Ntau-methyl-L-histidine
-
 ?
2.1.1.85 S-adenosyl-L-methionine + protein L-histidine negligible activity with: bovine serum albumin, fibrinogen, casein, histone type IIA, gelatin, human gamma-globulin Oryctolagus cuniculus S-adenosyl-L-homocysteine + protein Ntau-methyl-L-histidine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.85 37
-
 assay at Oryctolagus cuniculus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.85 40
-
 10 min, 75% loss of activity Oryctolagus cuniculus
2.1.1.85 50
-
 10 min, complete loss of activity Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.85 8
-
-
 Oryctolagus cuniculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.85 6.8 9 about 50% of activity maximum at pH 6.8 and 9.0 Oryctolagus cuniculus