Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zubieta, C.; Kota, P.; Ferrer, J.L.; Dixon, R.A.; Noel, J.P.
    Structural basis for the modulation of lignin monomer methylation by caffeic acid/5-hydroxyferulic acid 3/5-O-methyltransferase (2002), Plant Cell, 14, 1265-1277.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.68 expression in Escherichia coli BL21 Medicago sativa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.68 crystallization from polyethylene glycol solution, 2.2 A resolution, complex with S-adenosyl-L-homocysteine and ferulic acid Medicago sativa
2.1.1.68 crystallization from polyethylene glycol solution, 2.4 A resolution, complex with S-adenosyl-L-homocysteine and 5-hydroxyconiferaldehyde Medicago sativa

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.68 A162T same reactivity as wild type Medicago sativa
2.1.1.68 F172Y no reaction with caffeic acid, with other substrates same reactivity as wild type Medicago sativa
2.1.1.68 H183K no reaction with caffeic acid, with other substrates same reactivity as wild type Medicago sativa
2.1.1.68 L136Y same reactivity as wild type Medicago sativa
2.1.1.68 M130L no reaction with caffeic acid and 5-hydroxyferulic acid, with other substrates same reactivity as wild type Medicago sativa
2.1.1.68 N131K same reactivity as wild type Medicago sativa
2.1.1.68 N324Y no reaction with caffeic acid, with other substrates same reactivity as wild type Medicago sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.68 0.005
-
 5-hydroxyconiferaldehyde wild type Medicago sativa
2.1.1.68 0.0075
-
 5-hydroxyconiferyl alcohol wild type Medicago sativa
2.1.1.68 0.01
-
 5-hydroxyferulic acid wild type Medicago sativa
2.1.1.68 0.01
-
 caffeoyl aldehyde wild type Medicago sativa
2.1.1.68 0.013
-
 caffeoyl alcohol wild type Medicago sativa
2.1.1.68 0.043
-
 caffeic acid wild type Medicago sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.68 S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate Medicago sativa i.e. caffeic acid, involved in the biosynthesis of lignin S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.68 Medicago sativa P28002
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.68 S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
-
 Medicago sativa S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
 ?
2.1.1.68 S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate i.e. caffeic acid, involved in the biosynthesis of lignin Medicago sativa S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
 ?
2.1.1.68 S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde
-
 Medicago sativa S-adenosyl-L-homocysteine + sinapaldehyde
-
 ?
2.1.1.68 S-adenosyl-L-methionine + 5-hydroxyconiferyl alcohol
-
 Medicago sativa S-adenosyl-L-homocysteine + 5-hydroxy-3-methoxyconiferyl alcohol
-
 ?
2.1.1.68 S-adenosyl-L-methionine + 5-hydroxyferulic acid
-
 Medicago sativa S-adenosyl-L-homocysteine + sinapic acid
-
 ?
2.1.1.68 S-adenosyl-L-methionine + caffeoyl alcohol
-
 Medicago sativa S-adenosyl-L-homocysteine + 4-[(1E)-3-hydroxyprop-1-en-1-yl]-2-methoxyphenol
-
 ?
2.1.1.68 S-adenosyl-L-methionine + caffeoyl aldehyde
-
 Medicago sativa S-adenosyl-L-homocysteine + coniferyl aldehyde
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.68 COMT
-
 Medicago sativa