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Literature summary extracted from

  • Thompson, J.; Cundliffe, E.
    Purification and properties of an RNA methylase produced by Streptomyces azureus and involved in resistance to thiostrepton (1981), J. Gen. Microbiol., 124, 291-297.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.230 S-adenosyl-L-homocysteine
-
 Streptomyces azureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.230 0.15
-
 S-adenosyl-L-methionine pH 7.5, 35°C Streptomyces azureus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.230 K+ ionic optimum: 50-100 mM Streptomyces azureus
2.1.1.230 Mg2+ ionic optimum: 5-20 mM Streptomyces azureus
2.1.1.230 NH4+ ionic optimum: 50-100 mM Streptomyces azureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.230 35000 38000 gel filtration Streptomyces azureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.230 S-adenosyl-L-methionine + adenosine1067 in 23S rRNA Streptomyces azureus the enzyme is involved in resistance to thiostrepton S-adenosyl-L-homocysteine + 2'-O-methyladenosine1067 in 23S rRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.230 Streptomyces azureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.230
-
 Streptomyces azureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.230 S-adenosyl-L-methionine + adenosine1067 in 23S rRNA
-
 Streptomyces azureus S-adenosyl-L-homocysteine + 2'-O-methyladenosine1067 in 23S rRNA
-
 ?
2.1.1.230 S-adenosyl-L-methionine + adenosine1067 in 23S rRNA the enzyme is involved in resistance to thiostrepton Streptomyces azureus S-adenosyl-L-homocysteine + 2'-O-methyladenosine1067 in 23S rRNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.230 thiostrepton-resistance methylase
-
 Streptomyces azureus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.230 35
-
 assay at Streptomyces azureus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.1.1.230 30 45 quite active from 30°C-45°C Streptomyces azureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.230 7.5 7.6
-
 Streptomyces azureus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.230 7 7.8 pH 7.0: about 60% of maximal activity, pH 7.8: about 60% of maximal activity Streptomyces azureus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.1.1.230 6
-
 unstable below Streptomyces azureus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.230 0.0005
-
 S-adenosyl-L-homocysteine pH 7.5, 35°C Streptomyces azureus

General Information

EC Number General Information Comment Organism
2.1.1.230 physiological function the enzyme is involved in resistance to thiostrepton Streptomyces azureus