Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Martin, S.A.; Moss, B.
    mRNA guanylyltransferase and mRNA (guanine-7-)-methyltransferase from vaccinia virions. Donor and acceptor substrate specificites (1976), J. Biol. Chem., 251, 7313-7321.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.56 viral nucleocapsid
-
 Vaccinia virus 19013
-
2.7.7.50 viral nucleocapsid
-
 Vaccinia virus 19013
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.56 Mg2+ not required Vaccinia virus

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.56 Vaccinia virus
-
 from infected HeLa cells
-
2.1.1.56 Vaccinia virus WR
-
 from infected HeLa cells
-
2.7.7.50 Vaccinia virus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.56 from infected HeLa cells Vaccinia virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.56 additional information ATP, XTP, CTP, UTP, and guanosine containing compounds with phospho groups in either position 2' or 3' or in 3'-5'phosphodiester linkage Vaccinia virus ?
-
 ?
2.1.1.56 additional information ATP, XTP, CTP, UTP, and guanosine containing compounds with phospho groups in either position 2' or 3' or in 3'-5'phosphodiester linkage Vaccinia virus WR ?
-
 ?
2.1.1.56 S-adenosyl-L-methionine + G(5')pppN N: A Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppG
-
 ?
2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA acceptor substrate specificity, overview Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ?
2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA utilizes GMP, GDP, guanosine, ITP as substrates Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ?
2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA mRNA, synthetic polyribonucleotides terminating in a dinucleoside triphosphate Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ?
2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA specific for mRNAs with 5'-terminal sequence G(5')pppA- and G(5')pppG- Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ?
2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA R may be guanosine or adenosine, methyl acceptors are unmethylated vaccinia virus mRNAs Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ?
2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA acceptor substrate specificity, overview Vaccinia virus WR S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ?
2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA utilizes GMP, GDP, guanosine, ITP as substrates Vaccinia virus WR S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ?
2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA mRNA, synthetic polyribonucleotides terminating in a dinucleoside triphosphate Vaccinia virus WR S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ?
2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA specific for mRNAs with 5'-terminal sequence G(5')pppA- and G(5')pppG- Vaccinia virus WR S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ?
2.1.1.56 S-adenosyl-L-methionine + GTP
-
 Vaccinia virus S-adenosyl-L-homocysteine + m7GTP
-
 ?
2.7.7.50 dGTP + pp(5')RNA
-
 Vaccinia virus dG(5')ppp(5')RNA + diphosphate deoxyguanosine residue linked 5' through three phosphates to the 5' position of the terminal residue r
2.7.7.50 GTP + pp(5')RNA
-
 Vaccinia virus G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue r
2.7.7.50 additional information specificity overview Vaccinia virus ?
-
 ?
2.7.7.50 additional information lacks strict sequence specificity, homoribonucleotides containing purines are preferred, in presence of diphosphate the enzyme catalyzes the phosphorolysis of the dinucleoside triphosphate G(5')pp(5')A but not of m7(5')pp(5')A Vaccinia virus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.56 37
-
 assay at Vaccinia virus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.56 7.8
-
 assay at Vaccinia virus