EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.53 | cysteine | slight activatory effect | Nicotiana tabacum |
EC Number | General Stability | Organism |
---|---|---|
2.1.1.53 | 2-mercaptoethanol stabilizes | Nicotiana tabacum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.53 | Ag+ | 0.1 mM, strong | Nicotiana tabacum | |
2.1.1.53 | p-chloromercuribenzoate | strong | Nicotiana tabacum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.53 | 0.11 | - |
S-adenosyl-L-methionine | - |
Nicotiana tabacum | |
2.1.1.53 | 0.4 | - |
putrescine | - |
Nicotiana tabacum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.53 | additional information | Mg2+, Mn2+ and EDTA at 0.001 M have no influence | Nicotiana tabacum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.53 | 60000 | - |
gel filtration | Nicotiana tabacum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.53 | S-adenosyl-L-methionine + putrescine | Nicotiana tabacum | enzyme is probably involved in nicotine biosynthesis | S-adenosyl-L-homocysteine + N-methylputrescine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.53 | Nicotiana tabacum | - |
Bright Yellow | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.53 | - |
Nicotiana tabacum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.1.53 | root | roots of decapitated plants show highest activity, no activity in leaves | Nicotiana tabacum | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.1.1.53 | 0.051 | - |
putrescine | Nicotiana tabacum |
EC Number | Storage Stability | Organism |
---|---|---|
2.1.1.53 | -20°C, 0.05 M phosphate buffer, pH 7.5, 0.01 M 2-mercaptoethanol, 12.5% glucose, stable for several weeks | Nicotiana tabacum |
2.1.1.53 | 0°C, without addition of a sulfhydryl compound, most activity is lost within a few days | Nicotiana tabacum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.53 | S-adenosyl-L-methionine + N-methylputrescine | about 8% of activity compared to putrescine | Nicotiana tabacum | S-adenosylhomocysteine + N,N-dimethylputrescine | - |
? | |
2.1.1.53 | S-adenosyl-L-methionine + putrescine | - |
Nicotiana tabacum | S-adenosyl-L-homocysteine + N-methylputrescine | - |
? | |
2.1.1.53 | S-adenosyl-L-methionine + putrescine | enzyme is probably involved in nicotine biosynthesis | Nicotiana tabacum | S-adenosyl-L-homocysteine + N-methylputrescine | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.53 | 26 | - |
assay at | Nicotiana tabacum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.53 | 8 | 9 | - |
Nicotiana tabacum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.53 | additional information | no requirement of cofactors | Nicotiana tabacum |