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Literature summary extracted from

  • Rao, M.M.; Rebello, P.F.; Pogell, B.M.
    Biosynthesis of puromycin in Streptomyces alboniger. Enzymatic methylation of O-demethylpuromycin (1969), J. Biol. Chem., 244, 112-118.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.38 medicine product puromycin is an antibiotic and antitumor agent Streptomyces alboniger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.38 0.01
-
 S-adenosyl-L-methionine
-
 Streptomyces alboniger
2.1.1.38 0.21
-
 O-demethylpuromycin
-
 Streptomyces alboniger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.38 68000
-
 sucrose density gradient centrifugation Streptomyces alboniger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.38 S-adenosyl-L-methionine + O-demethylpuromycin Streptomyces alboniger
-
 S-adenosyl-L-homocysteine + puromycin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.38 Streptomyces alboniger
-
-
-

Storage Stability

EC Number Storage Stability Organism
2.1.1.38 -65°C, can be stored for several months without loss of activity Streptomyces alboniger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.38 additional information L-tyrosine, L-tyrosine ethyl ester, L-tyrosine amide, catechol and epinephrine are not substrates Streptomyces alboniger ?
-
 ?
2.1.1.38 S-adenosyl-L-methionine + O-demethylpuromycin
-
 Streptomyces alboniger S-adenosyl-L-homocysteine + puromycin
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.38 7 9
-
 Streptomyces alboniger
2.1.1.38 8
-
 broad pH optimum over the range 7.0-9.0 with a maximum at pH 8.0 Streptomyces alboniger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.38 6 9
-
 Streptomyces alboniger