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Literature summary extracted from

  • Akamatsu, Y.; Law, J.H.
    The enzymatic synthesis of fatty acid methyl esters by carboxyl group alkylation (1970), J. Biol. Chem., 245, 709-713.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.15 Ca2+ slight inhibition Mycolicibacterium phlei
2.1.1.15 S-adenosylhomocysteine
-
 Mycolicibacterium phlei
2.1.1.15 unsaturated fatty acids
-
 Mycolicibacterium phlei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.15 0.025
-
 S-adenosylmethionine
-
 Mycolicibacterium phlei
2.1.1.15 1.3
-
 oleic acid
-
 Mycolicibacterium phlei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.15 S-adenosyl-L-methionine + a fatty acid Mycolicibacterium phlei synthesis of 10-methylstearic acid S-adenosyl-L-homocysteine + a fatty acid methyl ester
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.15 Mycolicibacterium phlei
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.15 additional information traces of activity with methionine, serine and S-adenosylethinonine as methyl donor Mycolicibacterium phlei ?
-
 ?
2.1.1.15 S-adenosyl-L-methionine + a fatty acid oleic acid is the most effective fatty acid acceptor, traces of activity with phosphatidylethanolamine and phosphatidylglycerol Mycolicibacterium phlei S-adenosyl-L-homocysteine + a fatty acid methyl ester
-
 ?
2.1.1.15 S-adenosyl-L-methionine + a fatty acid synthesis of 10-methylstearic acid Mycolicibacterium phlei S-adenosyl-L-homocysteine + a fatty acid methyl ester
-
 ?
2.1.1.15 S-adenosyl-L-methionine + oleic acid oleic acid is the most effective fatty acid acceptor, traces of activity with phosphatidylethanolamine and phosphatidylglycerol Mycolicibacterium phlei S-adenosyl-L-homocysteine + oleic acid methyl ester
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.15 50
-
 20% loss of activity within 5 min Mycolicibacterium phlei
2.1.1.15 100
-
 90% loss of activity within 10 min Mycolicibacterium phlei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.15 8 9
-
 Mycolicibacterium phlei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.15 7 10
-
 Mycolicibacterium phlei