BRENDA - Enzyme Database

The formation of corydaline and related alkaloids in Corydalis cava in vivo and in vitro

Rueffer, M.; Bauer, W.; Zenk, M.H.; Can. J. Chem. 72, 170-175 (1994)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.147
S-adenosyl-L-homocysteine
-
Corydalis cava
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.147
0.0027
-
S-adenosyl-L-methionine
-
Corydalis cava
2.1.1.147
0.005
-
Berberine
-
Corydalis cava
2.1.1.147
0.016
-
NADPH
-
Corydalis cava
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.1.147
cytosol
-
Corydalis cava
5829
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.147
56000
-
gel filtration
Corydalis cava
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.147
Corydalis cava
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.1.147
-
Corydalis cava
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.147
bulb
-
Corydalis cava
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.1.147
additional information
-
-
Corydalis cava
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.147
S-adenosyl-L-methionine + berberine + NADPH + H+
-
485156
Corydalis cava
S-adenosyl-L-homocysteine + corydaline + NADP+
-
485156
Corydalis cava
?
2.1.1.147
S-adenosyl-L-methionine + columbamine + NADPH + H+
27% of the activity with berberine
485156
Corydalis cava
S-adenosyl-L-homocysteine + isocorypalmine + NADP+
-
-
-
?
2.1.1.147
S-adenosyl-L-methionine + coptisine + NADPH + H+
21% of the activity with berberine
485156
Corydalis cava
S-adenosyl-L-homocysteine + tetrahydrocorysamine + NADP+
-
-
-
?
2.1.1.147
S-adenosyl-L-methionine + dihydroberberine + NADPH + H+
93% of the activity with berberine
485156
Corydalis cava
S-adenosyl-L-homocysteine + thalictricavine + NADP+
-
485156
Corydalis cava
?
2.1.1.147
S-adenosyl-L-methionine + jatrorrhizine + NADPH + H+
67% of the activity with berberine
485156
Corydalis cava
S-adenosyl-L-homocysteine + 7,8-dehydrothalictricavine + NADP+
-
-
-
?
2.1.1.147
S-adenosyl-L-methionine + palmatine + NADPH + H+
93% of the activity with berberine
485156
Corydalis cava
S-adenosyl-L-homocysteine + corydaline + NADP+
-
485156
Corydalis cava
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.1.1.147
37
-
-
Corydalis cava
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.1.1.147
20
50
20°C: about 35% of maximal activity, 50°C: about 75% of maximal activity
Corydalis cava
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.147
8
-
-
Corydalis cava
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.1.1.147
7
9.5
pH 7.0: about 45% of maximal activity, pH 9.5: about 30% of maximal activity
Corydalis cava
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.1.1.147
0.0026
-
S-adenosyl-L-homocysteine
-
Corydalis cava
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.147
S-adenosyl-L-homocysteine
-
Corydalis cava
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.1.1.147
0.0026
-
S-adenosyl-L-homocysteine
-
Corydalis cava
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.147
0.0027
-
S-adenosyl-L-methionine
-
Corydalis cava
2.1.1.147
0.005
-
Berberine
-
Corydalis cava
2.1.1.147
0.016
-
NADPH
-
Corydalis cava
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.1.147
cytosol
-
Corydalis cava
5829
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.147
56000
-
gel filtration
Corydalis cava
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.147
-
Corydalis cava
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.147
bulb
-
Corydalis cava
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.1.147
additional information
-
-
Corydalis cava
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.147
S-adenosyl-L-methionine + berberine + NADPH + H+
-
485156
Corydalis cava
S-adenosyl-L-homocysteine + corydaline + NADP+
-
485156
Corydalis cava
?
2.1.1.147
S-adenosyl-L-methionine + columbamine + NADPH + H+
27% of the activity with berberine
485156
Corydalis cava
S-adenosyl-L-homocysteine + isocorypalmine + NADP+
-
-
-
?
2.1.1.147
S-adenosyl-L-methionine + coptisine + NADPH + H+
21% of the activity with berberine
485156
Corydalis cava
S-adenosyl-L-homocysteine + tetrahydrocorysamine + NADP+
-
-
-
?
2.1.1.147
S-adenosyl-L-methionine + dihydroberberine + NADPH + H+
93% of the activity with berberine
485156
Corydalis cava
S-adenosyl-L-homocysteine + thalictricavine + NADP+
-
485156
Corydalis cava
?
2.1.1.147
S-adenosyl-L-methionine + jatrorrhizine + NADPH + H+
67% of the activity with berberine
485156
Corydalis cava
S-adenosyl-L-homocysteine + 7,8-dehydrothalictricavine + NADP+
-
-
-
?
2.1.1.147
S-adenosyl-L-methionine + palmatine + NADPH + H+
93% of the activity with berberine
485156
Corydalis cava
S-adenosyl-L-homocysteine + corydaline + NADP+
-
485156
Corydalis cava
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.1.1.147
37
-
-
Corydalis cava
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.1.1.147
20
50
20°C: about 35% of maximal activity, 50°C: about 75% of maximal activity
Corydalis cava
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.147
8
-
-
Corydalis cava
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.1.1.147
7
9.5
pH 7.0: about 45% of maximal activity, pH 9.5: about 30% of maximal activity
Corydalis cava