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Literature summary extracted from

  • Rueffer, M.; Bauer, W.; Zenk, M.H.
    The formation of corydaline and related alkaloids in Corydalis cava in vivo and in vitro (1994), Can. J. Chem., 72, 170-175.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.147 S-adenosyl-L-homocysteine
-
 Corydalis cava

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.147 0.0027
-
 S-adenosyl-L-methionine
-
 Corydalis cava
2.1.1.147 0.005
-
 Berberine
-
 Corydalis cava
2.1.1.147 0.016
-
 NADPH
-
 Corydalis cava

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.147 cytosol
-
 Corydalis cava 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.147 56000
-
 gel filtration Corydalis cava

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.147 Corydalis cava
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.147
-
 Corydalis cava

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.147 bulb
-
 Corydalis cava
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.147 additional information
-
-
 Corydalis cava

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.147 S-adenosyl-L-methionine + berberine + NADPH + H+
-
 Corydalis cava S-adenosyl-L-homocysteine + corydaline + NADP+
-
 ?
2.1.1.147 S-adenosyl-L-methionine + columbamine + NADPH + H+ 27% of the activity with berberine Corydalis cava S-adenosyl-L-homocysteine + isocorypalmine + NADP+
-
 ?
2.1.1.147 S-adenosyl-L-methionine + coptisine + NADPH + H+ 21% of the activity with berberine Corydalis cava S-adenosyl-L-homocysteine + tetrahydrocorysamine + NADP+
-
 ?
2.1.1.147 S-adenosyl-L-methionine + dihydroberberine + NADPH + H+ 93% of the activity with berberine Corydalis cava S-adenosyl-L-homocysteine + thalictricavine + NADP+
-
 ?
2.1.1.147 S-adenosyl-L-methionine + jatrorrhizine + NADPH + H+ 67% of the activity with berberine Corydalis cava S-adenosyl-L-homocysteine + 7,8-dehydrothalictricavine + NADP+
-
 ?
2.1.1.147 S-adenosyl-L-methionine + palmatine + NADPH + H+ 93% of the activity with berberine Corydalis cava S-adenosyl-L-homocysteine + corydaline + NADP+
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.147 37
-
-
 Corydalis cava

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.1.1.147 20 50 20°C: about 35% of maximal activity, 50°C: about 75% of maximal activity Corydalis cava

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.147 8
-
-
 Corydalis cava

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.147 7 9.5 pH 7.0: about 45% of maximal activity, pH 9.5: about 30% of maximal activity Corydalis cava

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.147 0.0026
-
 S-adenosyl-L-homocysteine
-
 Corydalis cava