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Literature summary extracted from

  • Woodcock, S.C.; Raux, E.; Levillayer, F.; Thermes, C.; Rambach, A.; Warren, M.J.
    Effect of mutations in the transmethylase and dehydrogenase/chelatase domains of siroheme synthase (CysG) on siroheme and cobalamin biosynthesis (1998), Biochem. J., 330, 121-129.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.107 complementation experiments with cysteine autotroph strain Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.107 D227A full activity Escherichia coli
2.1.1.107 D248A no transmethylase activity Escherichia coli
2.1.1.107 D303A full activity Escherichia coli
2.1.1.107 G224A unable to bind S-adenosyl-L-methionine Escherichia coli
2.1.1.107 K270I full activity Escherichia coli
2.1.1.107 R298L unable to bind S-adenosyl-L-methionine Escherichia coli
2.1.1.107 R309L no transmethylase activity Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.107 2 S-adenosyl-L-methionine + uroporphyrinogen III Escherichia coli involved in biosynthesis of cobalamin 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ product is precorrin-2 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.107 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.107
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.107 2 S-adenosyl-L-methionine + uroporphyrinogen III methylates positions 2 and 7 Escherichia coli 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ product is precorrin-2 ?
2.1.1.107 2 S-adenosyl-L-methionine + uroporphyrinogen III involved in biosynthesis of cobalamin Escherichia coli 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ product is precorrin-2 ?