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Literature summary extracted from

  • Buckner, F.S.; Kateete, D.P.; Lubega, G.W.; Van Voorhis, W.C.; Yokoyama, K.
    Trypanosoma brucei prenylated-protein carboxyl methyltransferase prefers farnesylated substrates (2002), Biochem. J., 367, 809-816.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.100 expression in Sf9 cells Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.100 n-octyl beta-glucoside 0.2%, 55-75% inhibition Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.100 0.00017
-
 N-acetyl-S-(E,E)-farnesyl-L-cysteine recombinant enzyme Trypanosoma brucei
2.1.1.100 0.0014
-
 N-acetyl-S-(E,E)-farnesyl-L-cysteine native enzyme Trypanosoma brucei
2.1.1.100 0.0023
-
 N-acetyl-S-geranylgeranyl-L-cysteine recombinant enzyme Trypanosoma brucei
2.1.1.100 0.004
-
 N-acetyl-S-geranylgeranyl-L-cysteine native enzyme Trypanosoma brucei
2.1.1.100 0.0115
-
 N-acetyl-S-(E,E)-farnesyl-L-cysteine native enzyme Mus musculus
2.1.1.100 0.0116
-
 N-acetyl-S-geranylgeranyl-L-cysteine native enzyme Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.100 membrane
-
 Mus musculus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.100 28301
-
 x * 28301, deduced from nucleotide sequence Trypanosoma brucei

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.100 Mus musculus
-
-
-
2.1.1.100 Trypanosoma brucei
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.100 liver
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.100 S-adenosyl-L-methionine + N-acetyl-S-geranylgeranyl-L-cysteine
-
 Mus musculus S-adenosyl-L-homocysteine + N-acetyl-S-geranylgeranyl-L-cysteine methyl ester
-
 ?
2.1.1.100 S-adenosyl-L-methionine + N-acetyl-S-geranylgeranyl-L-cysteine
-
 Trypanosoma brucei S-adenosyl-L-homocysteine + N-acetyl-S-geranylgeranyl-L-cysteine methyl ester
-
 ?
2.1.1.100 S-adenosyl-L-methionine + protein C-terminal S-farnesyl-L-cysteine
-
 Mus musculus S-adenosyl-L-homocysteine + protein C-terminal S-farnesyl-L-cysteine methyl ester
-
 ?
2.1.1.100 S-adenosyl-L-methionine + protein C-terminal S-farnesyl-L-cysteine
-
 Trypanosoma brucei S-adenosyl-L-homocysteine + protein C-terminal S-farnesyl-L-cysteine methyl ester
-
 ?
2.1.1.100 S-adenosylmethionine + N-acetyl-S-(E,E)-farnesyl-L-cysteine
-
 Mus musculus S-adenosylhomocysteine + N-acetyl-S-(E,E)-farnesyl-L-cysteine methyl ester
-
 ?
2.1.1.100 S-adenosylmethionine + N-acetyl-S-(E,E)-farnesyl-L-cysteine recombinant enzyme: 50fold higher activity than with N-acetyl-S-geranylgeranyl-L-cysteine, native enzyme: 40fold higher activity than with N-acetyl-S-geranylgeranyl-L-cysteine Trypanosoma brucei S-adenosylhomocysteine + N-acetyl-S-(E,E)-farnesyl-L-cysteine methyl ester
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.100 ? x * 28301, deduced from nucleotide sequence Trypanosoma brucei