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Literature summary extracted from

  • Zhang, H.P.; Yang, N.; Armarego, W.L.F.
    In vitro mutagenesis of human dihydropteridine reductase at the active site and at altered sites found in the reductases of deficient children (1996), Pteridines, 7, 123-136.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.34 expression of wild-type, Y150H, Y150S, Y150F, Y150E, Y150K, G151S and G23D in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.5.1.34 G151S mutation found in children suffering from phenylketonuria due to enzyme deficiency Homo sapiens
1.5.1.34 G23D mutation found in children suffering from phenylketonuria due to enzyme deficiency, inactive mutant enzyme Homo sapiens
1.5.1.34 Y150E lower kcat with 6-methyl-7,8-dyhydropterin than wild-type Homo sapiens
1.5.1.34 Y150F lower kcat with 6-methyl-7,8-dyhydropterin than wild-type Homo sapiens
1.5.1.34 Y150H lower kcat with 6-methyl-7,8-dyhydropterin than wild-type Homo sapiens
1.5.1.34 Y150K lower kcat with 6-methyl-7,8-dyhydropterin than wild-type Homo sapiens
1.5.1.34 Y150S lower kcat with 6-methyl-7,8-dyhydropterin than wild-type Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.34 0.0009
-
 quinonoid-7,8-dihydrobiopterin recombinant wild-type enzyme Homo sapiens
1.5.1.34 0.0009
-
 quinonoid 7,8-dihydropterin recombinant wild-type enzyme Homo sapiens
1.5.1.34 0.002
-
 NADH recombinant Y150S glutathione-S-transferase fusion mutant enzyme Homo sapiens
1.5.1.34 0.002
-
 NADH recombinant Y150K glutathione-S-transferase fusion mutant enzyme Homo sapiens
1.5.1.34 0.0024
-
 NADH recombinant Y150F mutant enzyme Homo sapiens
1.5.1.34 0.004
-
 quinonoid-7,8-dihydrobiopterin recombinant Y150H mutant enzyme Homo sapiens
1.5.1.34 0.0043
-
 NADH recombinant Y150H mutant enzyme Homo sapiens
1.5.1.34 0.0044
-
 NADH recombinant Y150E glutathione-S-transferase fusion mutant enzyme Homo sapiens
1.5.1.34 0.0049
-
 NADH recombinant Y150S mutant enzyme Homo sapiens
1.5.1.34 0.0049
-
 quinonoid 6-methyl-7,8-dihydropterin recombinant wild-type glutathione-S-transferase fusion enzyme Homo sapiens
1.5.1.34 0.005
-
 NADH recombinant Y150F glutathione-S-transferase fusion mutant enzyme Homo sapiens
1.5.1.34 0.0059
-
 NADH recombinant Y150H glutathione-S-transferase fusion mutant enzyme Homo sapiens
1.5.1.34 0.0062
-
 quinonoid 6-methyl-7,8-dihydropterin recombinant G151S mutant enzyme Homo sapiens
1.5.1.34 0.0064
-
 NADH recombinant Y150E mutant enzyme Homo sapiens
1.5.1.34 0.0075
-
 quinonoid 7,8-dihydropterin recombinant G151S mutant enzyme Homo sapiens
1.5.1.34 0.0114
-
 NADH recombinant wild-type glutathione-S-transferase fusion enzyme Homo sapiens
1.5.1.34 0.0172
-
 NADH recombinant wild-type enzyme Homo sapiens
1.5.1.34 0.0181
-
 quinonoid 6-methyl-7,8-dihydropterin recombinant wild-type enzyme Homo sapiens
1.5.1.34 0.0305
-
 quinonoid-7,8-dihydrobiopterin recombinant Y150S mutant enzyme Homo sapiens
1.5.1.34 0.0593
-
 quinonoid-7,8-dihydrobiopterin recombinant Y150F mutant enzyme Homo sapiens
1.5.1.34 0.0819
-
 quinonoid 6-methyl-7,8-dihydropterin recombinant Y150H mutant enzyme Homo sapiens
1.5.1.34 0.0874
-
 quinonoid 6-methyl-7,8-dihydropterin recombinant Y150H glutathione-S-transferase fusion mutant enzyme Homo sapiens
1.5.1.34 0.0988
-
 quinonoid 6-methyl-7,8-dihydropterin recombinant G23D mutant enzyme Homo sapiens
1.5.1.34 0.144
-
 quinonoid 7,8-dihydropterin recombinant G23D mutant enzyme Homo sapiens
1.5.1.34 0.149
-
 quinonoid 6-methyl-7,8-dihydropterin recombinant Y150E glutathione-S-transferase fusion mutant enzyme Homo sapiens
1.5.1.34 0.165
-
 quinonoid 6-methyl-7,8-dihydropterin recombinant Y150F glutathione-S-transferase fusion mutant enzyme Homo sapiens
1.5.1.34 0.2
-
 NADH recombinant G23D mutant enzyme Homo sapiens
1.5.1.34 0.252
-
 quinonoid-7,8-dihydrobiopterin recombinant Y150E mutant enzyme Homo sapiens
1.5.1.34 0.276
-
 NADH recombinant G151S mutant enzyme Homo sapiens
1.5.1.34 0.283
-
 quinonoid 6-methyl-7,8-dihydropterin recombinant Y150S mutant enzyme Homo sapiens
1.5.1.34 0.33
-
 quinonoid 6-methyl-7,8-dihydropterin recombinant Y150K glutathione-S-transferase fusion mutant enzyme Homo sapiens
1.5.1.34 0.406
-
 quinonoid 6-methyl-7,8-dihydropterin recombinant Y150S glutathione-S-transferase fusion mutant enzyme Homo sapiens
1.5.1.34 0.434
-
 quinonoid 6-methyl-7,8-dihydropterin recombinant Y150E mutant enzyme Homo sapiens
1.5.1.34 0.48
-
 quinonoid 6-methyl-7,8-dihydropterin recombinant Y150F mutant enzyme Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.34 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.34 wild-type and recombinant Y150H, Y150S, Y150F, Y150E, Y150K, G151S and G23D mutant enzymes Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.34 6,7-dihydropteridine + NADH
-
 Homo sapiens (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin + NAD+
-
 ?
1.5.1.34 quinoid 6-methyl-7,8-dihydropterin + NADH + H+
-
 Homo sapiens 6-methyl-5,6,7,8-tetrahydropterin + NAD+
-
 r
1.5.1.34 quinoid dihydrobiopterin + NAD(P)H
-
 Homo sapiens tetrahydrobiopterin + NAD(P)+
-
 ?
1.5.1.34 quinonoid 6-methyl-7,8-dihydropterin + NADH + H+
-
 Homo sapiens 6-methyl-5,6,7,8-tetrahydropterin + NAD+
-
 r
1.5.1.34 quinonoid 7,8-dihydropterin + NADH + H+
-
 Homo sapiens 5,6,7,8-tetrahydropterin + NAD+
-
 r
1.5.1.34 quinonoid 7,8-dihydropterin + NADPH + H+
-
 Homo sapiens 5,6,7,8-tetrahydropterin + NADP+
-
 r
1.5.1.34 quinonoid dihydrobiopterin + NADH + H+
-
 Homo sapiens 6R-(1'R,2'R)-5,6,7,8-tetrahydrobiopterin + NAD+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.34 0.3
-
 quinoid 6-methyl-7,8-dihydropterin recombinant G23D mutant enzyme Homo sapiens
1.5.1.34 2.3
-
 quinoid 6-methyl-7,8-dihydropterin recombinant Y150E glutathione-S-transferase fusion mutant enzyme Homo sapiens
1.5.1.34 6.7
-
 quinoid 6-methyl-7,8-dihydropterin recombinant Y150K glutathione-S-transferase fusion mutant enzyme Homo sapiens
1.5.1.34 7.3
-
 quinoid 6-methyl-7,8-dihydropterin recombinant Y150E mutant enzyme Homo sapiens
1.5.1.34 7.7
-
 quinoid 6-methyl-7,8-dihydropterin recombinant Y150F glutathione-S-transferase fusion mutant enzyme Homo sapiens
1.5.1.34 15.3
-
 quinoid 6-methyl-7,8-dihydropterin recombinant Y150F mutant enzyme Homo sapiens
1.5.1.34 15.7
-
 quinoid 7,8-dihydropterin recombinant G23D mutant enzyme Homo sapiens
1.5.1.34 16.7
-
 quinoid 7,8-dihydrobiopterin recombinant Y150E mutant enzyme Homo sapiens
1.5.1.34 31
-
 quinoid 6-methyl-7,8-dihydropterin recombinant wild-type glutathione-S-transferase fusion enzyme Homo sapiens
1.5.1.34 35.8
-
 quinoid 6-methyl-7,8-dihydropterin recombinant Y150S mutant enzyme Homo sapiens
1.5.1.34 42
-
 quinoid 6-methyl-7,8-dihydropterin recombinant Y150S glutathione-S-transferase fusion mutant enzyme Homo sapiens
1.5.1.34 42.2
-
 quinoid 7,8-dihydrobiopterin recombinant Y150H mutant enzyme Homo sapiens
1.5.1.34 44.5
-
 quinoid 7,8-dihydrobiopterin recombinant wild-type enzyme Homo sapiens
1.5.1.34 44.5
-
 quinoid 7,8-dihydropterin recombinant wild-type enzyme Homo sapiens
1.5.1.34 49.6
-
 quinoid 6-methyl-7,8-dihydropterin recombinant Y150H mutant enzyme Homo sapiens
1.5.1.34 50.3
-
 quinoid 7,8-dihydrobiopterin recombinant Y150S mutant enzyme Homo sapiens
1.5.1.34 51.2
-
 quinoid 7,8-dihydropterin recombinant G151S mutant enzyme Homo sapiens
1.5.1.34 52.2
-
 quinoid 7,8-dihydrobiopterin recombinant Y150F mutant enzyme Homo sapiens
1.5.1.34 60
-
 quinoid 6-methyl-7,8-dihydropterin recombinant Y150H glutathione-S-transferase fusion mutant enzyme Homo sapiens
1.5.1.34 107
-
 quinoid 6-methyl-7,8-dihydropterin recombinant G151S mutant enzyme Homo sapiens
1.5.1.34 227
-
 quinoid 6-methyl-7,8-dihydropterin recombinant wild-type enzyme Homo sapiens