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Literature summary extracted from

  • Hirayama, K.; Nakanisi, N.; Sueoka, T.; Katoh, S.; Yamada, S.
    Dihydropteridine reductase and tetrahydropterin in Crithidia fasciculata cells (1980), Biochim. Biophys. Acta, 612, 337-343.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.5.1.34 not stable during gel filtration Crithidia fasciculata
1.5.1.34 unstable to (NH4)2SO4 precipitation Crithidia fasciculata

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.34 aminopterin 1 mM, 50% inhibition Crithidia fasciculata
1.5.1.34 methopterin 1 mM, 10% inhibition Crithidia fasciculata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.34 0.0028
-
 NADH
-
 Crithidia fasciculata
1.5.1.34 0.0088
-
 NADPH
-
 Crithidia fasciculata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.34 55000
-
 gel filtration Crithidia fasciculata

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.34 Crithidia fasciculata
-
-
-

Storage Stability

EC Number Storage Stability Organism
1.5.1.34 -80°C, 40 mM potassium phosphate buffer, pH 6.8, stable for at least a week Crithidia fasciculata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.34 quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H more specific for NADH, 50% activity with NADPH, no activity with biopterin and 7,8-dihydrobiopterin Crithidia fasciculata 5,6,7,8-tetrahydrobiopterin + NAD(P)+
-
 ?
1.5.1.34 quinonoid dihydrobiopterin + NADH + H+
-
 Crithidia fasciculata 5,6,7,8-tetrahydrobiopterin + NAD+
-
 r

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.5.1.34 6.5 8 unstable below and above Crithidia fasciculata

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.34 NADH utilizes both NADH and NADPH Crithidia fasciculata
1.5.1.34 NADH enzyme prefers NADH Crithidia fasciculata
1.5.1.34 NADPH utilizes both NADH and NADPH Crithidia fasciculata
1.5.1.34 NADPH NADH is preferred Crithidia fasciculata