EC Number | Application | Comment | Organism |
---|---|---|---|
3.2.1.31 | analysis | reporter enzyme which is used for studies in higher plants because endogenous activities are low and sensitive assays are available. A version of GUS with phenylalanine at the mature N-terminus accumulates a minimum of 3fold lower than GUS with methionine at its mature N-terminus. This altered protein can be useful for promoter studies which require more rapid changes in the accumulation of the reporter protein | Escherichia coli |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.12.7 | expression of a recombinant luciferase-ubiquitin enzyme in Saccharomyces cerevisiae | Photinus pyralis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.31 | additional information | the enzyme is engineered as fusion with an N-terminal 76 amino acid ubiquitin-coding region. When translated in any eukaryotic cell, such ubiquitin fusions are cleaved by ubiqitin-specific proteases specifically after the C-terminus of ubiquitin, irrespective of the distal amino acid (with the exception of Pro), releasing the downstream protein with the specified amino terminus. The presence of an N-terminal uncleavable ubiquitin on GUS does not reduce activity. A version of GUS with phenylalanine at the mature N-terminus accumulates a minimum of 3fold lower than GUS with methionine at its mature N-terminus | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.12.7 | Photinus pyralis | - |
- |
- |
3.2.1.31 | Escherichia coli | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.12.7 | Photinus luciferin + O2 + ATP | - |
Photinus pyralis | oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.31 | beta-glucuronidase | - |
Escherichia coli |
3.2.1.31 | GUS | - |
Escherichia coli |