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Literature summary extracted from
- Immobilization of firefly luciferase on glass rods: properties of the immobilized enzyme (1977), Anal. Biochem., 80, 496-501.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.12.7 | 0.002 | - |
ATP | immobilized enzyme | Photinus pyralis |  |
| 1.13.12.7 | 0.11 | - |
ATP | soluble and immobilized enzyme | Photinus pyralis | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.12.7 | Photinus pyralis | - |
- |
- |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.13.12.7 | 4°C, pH 7.0, 0.1 M phosphate, 1 mM 2-mercaptoethanol, immobilized, 3 weeks | Photinus pyralis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.12.7 | Photinus luciferin + O2 + ATP | - |
Photinus pyralis | oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.12.7 | additional information | - |
pH 7.3 with immobilized enzyme | Photinus pyralis |
| 1.13.12.7 | additional information | - |
pH 7.8 soluble enzyme | Photinus pyralis |
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Release 2023.1 (January 2023)
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