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Literature summary extracted from

  • DeLuca M.; McElroy, W.D.
    Two kinetically distinguishable ATP sites in firefly luciferase (1984), Biochem. Biophys. Res. Commun., 123, 764-770.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.12.7 ATP mechanism Photinus pyralis
1.13.12.7 Procion blue MX-R irreversible inhibitor, enzyme protected by luciferin, ATP and MgATP2-; time dependent Photinus pyralis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.12.7 0.11
-
 ATP two catalytically active sites: first site with a Km for ATP of 0.11 mM is responsible for initial flash, a second site with a Km for ATP of 0.02 mM catalyses the continuous low production of light Photinus pyralis

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.7 Photinus pyralis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.7 Photinus luciferin + O2 + ATP
-
 Photinus pyralis oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.12.7 dimer only one subunit is enzymatically active Photinus pyralis