BRENDA - Enzyme Database

Firefly luciferase

DeLuca, M.; Adv. Enzymol. Relat. Areas Mol. Biol. 44, 37-68 (1976)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.13.12.7
AMP
activator of yellow-green light at pH 6.5
Photinus pyralis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.13.12.7
2-(6'-hydroxy-2'-benzothiazolyl)-4-hydroxymethylthiazole
competitive inhibitor
Photinus pyralis
1.13.12.7
5-anilino-1-naphthalene sulfonate
-
Photinus pyralis
1.13.12.7
6-toluidino-2-naphthalene sulfonate
-
Photinus pyralis
1.13.12.7
ATP
uncomplexed ATP is a competitive inhibitor with respect to MgATP2- complex
Photinus pyralis
1.13.12.7
dehydroluciferin
-
Photinus pyralis
1.13.12.7
N-ethylmaleimide
-
Photinus pyralis
1.13.12.7
N-tosyl-L-phenylalanine chloromethyl ketone
-
Photinus pyralis
1.13.12.7
p-mercuribenzoate
-
Photinus pyralis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.13.12.7
Zn2+
increasing concentration shifts the light wavelenghts to higher values
Photinus pyralis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.12.7
50000
-
-
Photinus pyralis
1.13.12.7
92000
95000
high speed equilibrium sedimentation
Photinus pyralis
1.13.12.7
100000
-
from sedimentation velocity of the crystalline form
Photinus pyralis
1.13.12.7
500000
-
2 * 500000 sedimentation equilibrium study of guanidine hydrochloride-treated enzyme
Photinus pyralis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.13.12.7
Photinus luciferin + O2 + ATP
Photinus pyralis
highly specific for ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
Photinus pyralis
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.13.12.7
Photinus pyralis
-
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.13.12.7
additional information
-
quantum yield 0.88 with respect to luciferin
Photinus pyralis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.12.7
5,5'-dimethylluciferin + ATP + O2
produces only red light, 6-amino and 4 hydroxy analogs tested
484831
Photinus pyralis
dehydroluciferin-adenylate + diphosphate
-
484831
Photinus pyralis
?
1.13.12.7
dehydroluciferin + ATP + O2
-
484831
Photinus pyralis
diphosphate + dehydroluciferin-adenylate
-
484831
Photinus pyralis
?
1.13.12.7
luciferin + ATP + O2
-
484831
Photinus pyralis
diadenosine 5',5''-P1,P4-tetraphosphate
-
484831
Photinus pyralis
r
1.13.12.7
luciferin + MgATP2-
-
484831
Photinus pyralis
AMP + diphosphate
-
484831
Photinus pyralis
?
1.13.12.7
Photinus luciferin + O2 + ATP
highly specific for ATP
484831
Photinus pyralis
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
484831
Photinus pyralis
?
1.13.12.7
Photinus luciferin + O2 + ATP
the true substrate is MgATP2-
484831
Photinus pyralis
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
484831
Photinus pyralis
?
Subunits
EC Number
Subunits
Commentary
Organism
1.13.12.7
dimer
2 * 500000 sedimentation equilibrium study of guanidine hydrochloride-treated enzyme; two non identical subunits
Photinus pyralis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.13.12.7
7.5
8
pH below 7.5 emission shifts to longer wavelengths; pH up to 8 red light occurs instead of yellow-green light
Photinus pyralis
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.13.12.7
0.00001
-
dehydroluciferin
-
Photinus pyralis
1.13.12.7
0.0006
-
2-(6'-hydroxy-2'-benzothiazolyl)-4-hydroxymethylthiazole
-
Photinus pyralis
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.13.12.7
AMP
activator of yellow-green light at pH 6.5
Photinus pyralis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.13.12.7
2-(6'-hydroxy-2'-benzothiazolyl)-4-hydroxymethylthiazole
competitive inhibitor
Photinus pyralis
1.13.12.7
5-anilino-1-naphthalene sulfonate
-
Photinus pyralis
1.13.12.7
6-toluidino-2-naphthalene sulfonate
-
Photinus pyralis
1.13.12.7
ATP
uncomplexed ATP is a competitive inhibitor with respect to MgATP2- complex
Photinus pyralis
1.13.12.7
dehydroluciferin
-
Photinus pyralis
1.13.12.7
N-ethylmaleimide
-
Photinus pyralis
1.13.12.7
N-tosyl-L-phenylalanine chloromethyl ketone
-
Photinus pyralis
1.13.12.7
p-mercuribenzoate
-
Photinus pyralis
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.13.12.7
0.00001
-
dehydroluciferin
-
Photinus pyralis
1.13.12.7
0.0006
-
2-(6'-hydroxy-2'-benzothiazolyl)-4-hydroxymethylthiazole
-
Photinus pyralis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.13.12.7
Zn2+
increasing concentration shifts the light wavelenghts to higher values
Photinus pyralis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.12.7
50000
-
-
Photinus pyralis
1.13.12.7
92000
95000
high speed equilibrium sedimentation
Photinus pyralis
1.13.12.7
100000
-
from sedimentation velocity of the crystalline form
Photinus pyralis
1.13.12.7
500000
-
2 * 500000 sedimentation equilibrium study of guanidine hydrochloride-treated enzyme
Photinus pyralis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.13.12.7
Photinus luciferin + O2 + ATP
Photinus pyralis
highly specific for ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
Photinus pyralis
?
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.13.12.7
additional information
-
quantum yield 0.88 with respect to luciferin
Photinus pyralis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.12.7
5,5'-dimethylluciferin + ATP + O2
produces only red light, 6-amino and 4 hydroxy analogs tested
484831
Photinus pyralis
dehydroluciferin-adenylate + diphosphate
-
484831
Photinus pyralis
?
1.13.12.7
dehydroluciferin + ATP + O2
-
484831
Photinus pyralis
diphosphate + dehydroluciferin-adenylate
-
484831
Photinus pyralis
?
1.13.12.7
luciferin + ATP + O2
-
484831
Photinus pyralis
diadenosine 5',5''-P1,P4-tetraphosphate
-
484831
Photinus pyralis
r
1.13.12.7
luciferin + MgATP2-
-
484831
Photinus pyralis
AMP + diphosphate
-
484831
Photinus pyralis
?
1.13.12.7
Photinus luciferin + O2 + ATP
highly specific for ATP
484831
Photinus pyralis
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
484831
Photinus pyralis
?
1.13.12.7
Photinus luciferin + O2 + ATP
the true substrate is MgATP2-
484831
Photinus pyralis
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
484831
Photinus pyralis
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.13.12.7
dimer
2 * 500000 sedimentation equilibrium study of guanidine hydrochloride-treated enzyme; two non identical subunits
Photinus pyralis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.13.12.7
7.5
8
pH below 7.5 emission shifts to longer wavelengths; pH up to 8 red light occurs instead of yellow-green light
Photinus pyralis