Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • DeLuca, M.
    Firefly luciferase (1976), Adv. Enzymol. Relat. Areas Mol. Biol., 44, 37-68.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.12.7 AMP activator of yellow-green light at pH 6.5 Photinus pyralis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.12.7 2-(6'-hydroxy-2'-benzothiazolyl)-4-hydroxymethylthiazole competitive inhibitor Photinus pyralis
1.13.12.7 5-anilino-1-naphthalene sulfonate
-
 Photinus pyralis
1.13.12.7 6-toluidino-2-naphthalene sulfonate
-
 Photinus pyralis
1.13.12.7 ATP uncomplexed ATP is a competitive inhibitor with respect to MgATP2- complex Photinus pyralis
1.13.12.7 dehydroluciferin
-
 Photinus pyralis
1.13.12.7 N-ethylmaleimide
-
 Photinus pyralis
1.13.12.7 N-tosyl-L-phenylalanine chloromethyl ketone
-
 Photinus pyralis
1.13.12.7 p-mercuribenzoate
-
 Photinus pyralis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.12.7 Zn2+ increasing concentration shifts the light wavelenghts to higher values Photinus pyralis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.12.7 50000
-
-
 Photinus pyralis
1.13.12.7 92000 95000 high speed equilibrium sedimentation Photinus pyralis
1.13.12.7 100000
-
 from sedimentation velocity of the crystalline form Photinus pyralis
1.13.12.7 500000
-
 2 * 500000 sedimentation equilibrium study of guanidine hydrochloride-treated enzyme Photinus pyralis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.12.7 Photinus luciferin + O2 + ATP Photinus pyralis highly specific for ATP oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.7 Photinus pyralis
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.12.7 additional information
-
 quantum yield 0.88 with respect to luciferin Photinus pyralis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.7 5,5'-dimethylluciferin + ATP + O2 produces only red light, 6-amino and 4 hydroxy analogs tested Photinus pyralis dehydroluciferin-adenylate + diphosphate
-
 ?
1.13.12.7 dehydroluciferin + ATP + O2
-
 Photinus pyralis diphosphate + dehydroluciferin-adenylate
-
 ?
1.13.12.7 luciferin + ATP + O2
-
 Photinus pyralis oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv
-
 r
1.13.12.7 luciferin + MgATP2-
-
 Photinus pyralis AMP + diphosphate
-
 ?
1.13.12.7 Photinus luciferin + O2 + ATP highly specific for ATP Photinus pyralis oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
 ?
1.13.12.7 Photinus luciferin + O2 + ATP the true substrate is MgATP2- Photinus pyralis oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.12.7 dimer two non identical subunits Photinus pyralis
1.13.12.7 dimer 2 * 500000 sedimentation equilibrium study of guanidine hydrochloride-treated enzyme Photinus pyralis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.12.7 7.5 8 pH up to 8 red light occurs instead of yellow-green light Photinus pyralis
1.13.12.7 7.5 8 pH below 7.5 emission shifts to longer wavelengths Photinus pyralis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.13.12.7 0.00001
-
 dehydroluciferin
-
 Photinus pyralis
1.13.12.7 0.0006
-
 2-(6'-hydroxy-2'-benzothiazolyl)-4-hydroxymethylthiazole
-
 Photinus pyralis