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Literature summary extracted from

  • Toman, P.D.; Lynch, J.J.; Schmidt, R.R.
    Purification and partial characterization of ribulose bisphosphate carboxylase holoenzyme and its subunits from Chlorella sorokinia and use of its antigen affinity-purified antibodies in specific immunoprecipitation and immunoadsorption procedures (1985), Plant Physiol., 79, 806-814.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.39 cytoplasm
-
 Chlorella sorokiniana 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.39 Mg2+ activation Chlorella sorokiniana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.39 55000
-
 A8B8, 8 * 55000 (A) + 8 *15800 (B), SDS-PAGE Chlorella sorokiniana
4.1.1.39 562000
-
 gel filtration Chlorella sorokiniana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.39 D-Ribulose 1,5-bisphosphate + CO2 Chlorella sorokiniana
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.39 Chlorella sorokiniana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.39
-
 Chlorella sorokiniana

Reaction

EC Number Reaction Comment Organism Reaction ID
4.1.1.39 2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O D-ribulose 1,5-bisphosphate + CO2 = 2 3-phospho-D-glycerate, carboxylase reaction Chlorella sorokiniana
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.39 0.133
-
 carboxylase Chlorella sorokiniana

Storage Stability

EC Number Storage Stability Organism
4.1.1.39 -20°C, 2 mM DTT, 25 mM Tris-buffer pH 8.9, 190 mM Gly Chlorella sorokiniana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.39 D-Ribulose 1,5-bisphosphate + CO2
-
 Chlorella sorokiniana D-3-Phosphoglycerate
-
 ?
4.1.1.39 D-Ribulose 1,5-bisphosphate + CO2
-
 Chlorella sorokiniana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.39 hexadecamer A8B8, 8 * 55000 (A) + 8 *15800 (B), SDS-PAGE Chlorella sorokiniana