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Literature summary extracted from

  • Terzaghi, B.E.; Laing, W.A.; Christeller, J.T.; Petersen, G.B.; Hill, D.F.
    Ribulose 1,5-bisphosphate carboxylase. Effect on the catalytic properties of changing methionine-330 to leucine in the Rhodospirillum rubrum enzyme (1986), Biochem. J., 235, 839-846.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.39 expression in Escherichia coli Rhodospirillum rubrum

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.39 M330L increase of Km for carboxylase and oxygenase reaction Rhodospirillum rubrum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.39 0.05
-
 D-ribulose 1,5-bisphosphate wild-type enzyme Rhodospirillum rubrum
4.1.1.39 0.16
-
 CO2 wild-type enzyme Rhodospirillum rubrum
4.1.1.39 0.17
-
 O2 wild-type enzyme Rhodospirillum rubrum
4.1.1.39 1.4
-
 D-ribulose 1,5-bisphosphate mutant Met330Leu Rhodospirillum rubrum
4.1.1.39 2.35
-
 CO2 mutant Met330Leu Rhodospirillum rubrum
4.1.1.39 6
-
 O2 mutant Met330Leu Rhodospirillum rubrum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.39 D-Ribulose 1,5-bisphosphate + CO2 Rhodospirillum rubrum
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.39 Rhodospirillum rubrum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.39
-
 Rhodospirillum rubrum

Reaction

EC Number Reaction Comment Organism Reaction ID
4.1.1.39 2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O D-ribulose 1,5-bisphosphate + CO2 = 2 3-phospho-D-glycerate, carboxylase reaction Rhodospirillum rubrum
a
4.1.1.39 2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O D-Ribulose 1,5-bisphosphate + O2 = D-3-phosphoglycerate + 2-phosphoglycolate, oxygenase reaction Rhodospirillum rubrum
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.39 D-Ribulose 1,5-bisphosphate + CO2
-
 Rhodospirillum rubrum D-3-Phosphoglycerate
-
 ?
4.1.1.39 D-Ribulose 1,5-bisphosphate + CO2
-
 Rhodospirillum rubrum ?
-
 ?
4.1.1.39 D-Ribulose 1,5-bisphosphate + O2
-
 Rhodospirillum rubrum D-3-Phosphoglycerate + 2-phosphoglycolate
-
 ?