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Literature summary extracted from
- tRNA 3' processing in plants: nuclear and mitochondrial activities differ (2000), Biochemistry, 39, 2096-2105.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.26.11 | NH4+ | optimal processing at 2 mM, nuclear RNase Z | Triticum aestivum |  |
| 3.1.26.11 | NH4+ | optimal processing at 2 mM, nuclear RNase Z | Solanum tuberosum | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.26.11 | Ca2+ | more than 20 mM | Solanum tuberosum | |
| 3.1.26.11 | Ca2+ | more than 20 mM | Triticum aestivum | |
| 3.1.26.11 | EDTA | 10 mM | Solanum tuberosum | |
| 3.1.26.11 | EDTA | 10 mM | Triticum aestivum | |
| 3.1.26.11 | K+ | more than 150 mM | Solanum tuberosum | |
| 3.1.26.11 | K+ | more than 150 mM | Triticum aestivum | |
| 3.1.26.11 | Mg2+ | more than 50 mM | Solanum tuberosum | |
| 3.1.26.11 | Mg2+ | more than 50 mM | Triticum aestivum | |
| 3.1.26.11 | NH4+ | more than 100 mM | Solanum tuberosum | |
| 3.1.26.11 | NH4+ | more than 100 mM | Triticum aestivum | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.26.11 | mitochondrion | - |
Triticum aestivum | 5739 | - |
| 3.1.26.11 | mitochondrion | - |
Solanum tuberosum | 5739 | - |
| 3.1.26.11 | nucleus | - |
Triticum aestivum | 5634 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.26.11 | Ca2+ | optimal processing at 2 mM, nuclear RNase Z | Triticum aestivum | |
| 3.1.26.11 | Ca2+ | optimal processing at 2 mM, nuclear RNase Z | Solanum tuberosum | |
| 3.1.26.11 | K+ | - |
Solanum tuberosum | |
| 3.1.26.11 | K+ | 30 mM, mitochondrial RNase Z | Triticum aestivum | |
| 3.1.26.11 | Mg2+ | 5 mM, mitochondrial RNase Z | Triticum aestivum | |
| 3.1.26.11 | Mg2+ | 5 mM, mitochondrial RNase Z | Solanum tuberosum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.26.11 | 43000 | - |
x * 43000, mitochondrial RNase Z, SDS-PAGE | Triticum aestivum |
| 3.1.26.11 | 122000 | - |
gel filtration, nuclear RNase Z | Triticum aestivum |
| 3.1.26.11 | 165000 | - |
gel filtration, mitochondrial RNase Z | Triticum aestivum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.26.11 | Solanum tuberosum | - |
- |
- |
| 3.1.26.11 | Triticum aestivum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.26.11 | - |
Solanum tuberosum |
| 3.1.26.11 | partial | Triticum aestivum |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.26.11 | ? | x * 43000, mitochondrial RNase Z, SDS-PAGE | Triticum aestivum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.26.11 | 30 | - |
- |
Solanum tuberosum |
| 3.1.26.11 | 30 | - |
nuclear RNase Z | Triticum aestivum |
| 3.1.26.11 | 35 | - |
mitochondrial RNase Z | Triticum aestivum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.26.11 | 8 | - |
- |
Solanum tuberosum |
| 3.1.26.11 | 8 | - |
nuclear RNase Z | Triticum aestivum |
| 3.1.26.11 | 8.4 | - |
mitochondrial RNase Z | Triticum aestivum |
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Release 2023.1 (January 2023)
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