Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wang, Y.; Lee, G.F.; Kelley, R.F.; Spellman, M.W.
    Identification of a GDP-L-fucose:polypeptide fucosyltransferase and enzymic addition of O-linked fucose to EGF domains (1996), Glycobiology, 6, 837-842.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.68 membrane enzyme has a cytosolic part and a stem region which is very suceptible to proteolysis Cricetulus griseus 16020
-
2.4.1.68 membrane enzyme has a cytosolic part and a stem region which is very suceptible to proteolysis Rattus norvegicus 16020
-
2.4.1.221 membrane
-
 Cricetulus griseus 16020
-
2.4.1.221 membrane
-
 Rattus norvegicus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.68 Ca2+ activation Cricetulus griseus
2.4.1.68 Mn2+ activation, 10fold at 50 mM Cricetulus griseus
2.4.1.68 Mn2+ activation, 10fold at 50 mM Rattus norvegicus
2.4.1.68 additional information no effect of EDTA Cricetulus griseus
2.4.1.68 Ni2+ activation Cricetulus griseus
2.4.1.221 Ca2+ 2fold increase at 10 mM Cricetulus griseus
2.4.1.221 Ca2+ 2fold increase at 10 mM Rattus norvegicus
2.4.1.221 Mn2+ 10fold increase at 50 mM, 4fold increase at 10 mM Cricetulus griseus
2.4.1.221 Mn2+ 10fold increase at 50 mM, 4fold increase at 10 mM Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.68 Cricetulus griseus
-
-
-
2.4.1.68 Rattus norvegicus
-
-
-
2.4.1.221 Cricetulus griseus
-
 Chinese hamster, CHO cells
-
2.4.1.221 Rattus norvegicus
-
 rat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.68 partially from CHO cell culture Cricetulus griseus
2.4.1.221 partial Cricetulus griseus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.68 CHO cell
-
 Cricetulus griseus
-
2.4.1.68 liver
-
 Rattus norvegicus
-
2.4.1.221 CHO cell
-
 Cricetulus griseus
-
2.4.1.221 liver
-
 Cricetulus griseus
-
2.4.1.221 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.68 additional information
-
 assay method development Cricetulus griseus
2.4.1.68 additional information
-
 assay method development Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.4.1.68 -20°C, no loss of activity, at least 1 month Cricetulus griseus
2.4.1.68 -20°C, no loss of activity, at least 1 month Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.68 GDP-L-fucose + factor VII EGF-1 domain recombinant human acceptor substrate, with and without His-tag Cricetulus griseus GDP + L-fucose alpha-1,6 bound to the asparagine-linked N-acetylglucosamine of the factor VII EGF-1 domain fucose is attached through an o-glycosidic linkage to a conserved serine or threonine residue ?
2.4.1.68 GDP-L-fucose + factor VII EGF-1 domain recombinant human acceptor substrate, with and without His-tag Rattus norvegicus GDP + L-fucose alpha-1,6 bound to the asparagine-linked N-acetylglucosamine of the factor VII EGF-1 domain fucose is attached through an o-glycosidic linkage to a conserved serine or threonine residue ?
2.4.1.68 additional information synthetic peptides of 5 proteins which contain O-linked fucose do not serve as acceptor substrates, overview Cricetulus griseus ?
-
 ?
2.4.1.68 additional information synthetic peptides of 5 proteins which contain O-linked fucose do not serve as acceptor substrates, overview Rattus norvegicus ?
-
 ?
2.4.1.221 additional information links fucose through an O-glycosidic linkage to a conserved serine or threonine residue in of the EGF-1 domain of human factor VII, various synthetic peptides serve as substrates Cricetulus griseus ?
-
 ?
2.4.1.221 additional information links fucose through an O-glycosidic linkage to a conserved serine or threonine residue in of the EGF-1 domain of human factor VII, various synthetic peptides serve as substrates Rattus norvegicus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.68 37
-
 assay at Cricetulus griseus
2.4.1.68 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.68 7
-
 assay at Cricetulus griseus
2.4.1.68 7
-
 assay at Rattus norvegicus