EC Number | General Stability | Organism |
---|---|---|
2.1.2.1 | DTT stabilizes | Saccharomyces cerevisiae |
2.1.2.1 | pyridoxal 5'-phosphate stabilizes | Saccharomyces cerevisiae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.2.1 | additional information | not: EDTA; not: purine nucleoside mono-, di- and triphosphates | Saccharomyces cerevisiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.2.1 | additional information | - |
additional information | - |
Saccharomyces cerevisiae | |
2.1.2.1 | 0.15 | 0.9 | L-serine | - |
Saccharomyces cerevisiae |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.1.2.1 | Ca2+ | activation at 0.5-10 mM | Saccharomyces cerevisiae | |
2.1.2.1 | Mg2+ | activation at 0.5-10 mM | Saccharomyces cerevisiae | |
2.1.2.1 | Mn2+ | activation at 0.5-10 mM | Saccharomyces cerevisiae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.2.1 | 180000 | 200000 | gel filtration, sucrose density gradient centrifugation | Saccharomyces cerevisiae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.1 | L-serine + tetrahydrofolate | Saccharomyces cerevisiae | key enzyme of serine pathway for assimilation of C1-compounds | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
2.1.2.1 | L-serine + tetrahydrofolate | Saccharomyces cerevisiae 3701B | key enzyme of serine pathway for assimilation of C1-compounds | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.2.1 | Saccharomyces cerevisiae | - |
- |
- |
2.1.2.1 | Saccharomyces cerevisiae 3701B | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.2.1 | - |
Saccharomyces cerevisiae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.1.2.1 | additional information | - |
- |
Saccharomyces cerevisiae |
EC Number | Storage Stability | Organism |
---|---|---|
2.1.2.1 | -10°C, 50% loss of activity in 10 h | Saccharomyces cerevisiae |
2.1.2.1 | 4°C, 50% loss of activity in 10 h | Saccharomyces cerevisiae |
2.1.2.1 | frozen, up to a month in the presence of 30% v/v glycerol | Saccharomyces cerevisiae |
2.1.2.1 | glycerol, 30% v/v, enhances thermal stability | Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.1 | L-serine + tetrahydrofolate | - |
Saccharomyces cerevisiae | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
2.1.2.1 | L-serine + tetrahydrofolate | key enzyme of serine pathway for assimilation of C1-compounds | Saccharomyces cerevisiae | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
2.1.2.1 | L-serine + tetrahydrofolate | - |
Saccharomyces cerevisiae 3701B | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
2.1.2.1 | L-serine + tetrahydrofolate | key enzyme of serine pathway for assimilation of C1-compounds | Saccharomyces cerevisiae 3701B | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.2.1 | additional information | - |
glycerol, 30% v/v, enhances thermal stability | Saccharomyces cerevisiae |
2.1.2.1 | 55 | - |
t1/2: about 2 min, glycerol stabilizes | Saccharomyces cerevisiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.2.1 | 8 | 9.5 | - |
Saccharomyces cerevisiae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.2.1 | pyridoxal 5'-phosphate | - |
Saccharomyces cerevisiae | |
2.1.2.1 | tetrahydrofolate | requirement with L-serine or L-2-methylserine as substrate | Saccharomyces cerevisiae |