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Literature summary extracted from

  • Schirch, L.; Peterson, D.
    Purification and properties of mitochondrial serine hydroxymethyltransferase (1980), J. Biol. Chem., 255, 7801-7806.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.2.1 mitochondrial enzyme Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.1 Antibodies to cytosolic enzyme
-
 Oryctolagus cuniculus
2.1.2.1 Antibodies to mitochondrial enzyme no inhibition of cytosolic enzyme Oryctolagus cuniculus
2.1.2.1 L-cysteine
-
 Oryctolagus cuniculus
2.1.2.1 L-serine competitive to glycine Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.1 0.04 0.046 tetrahydrofolate
-
 Oryctolagus cuniculus
2.1.2.1 1.4
-
 L-serine
-
 Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.1 cytoplasm
-
 Oryctolagus cuniculus 5737
-
2.1.2.1 mitochondrion
-
 Oryctolagus cuniculus 5739
-
2.1.2.1 additional information comparison of cytosolic and mitochondrial forms of enzyme Oryctolagus cuniculus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.1 53000
-
 4 * 53000, SDS-PAGE Oryctolagus cuniculus
2.1.2.1 215000
-
 gel filtration, amino acid composition Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.1
-
 Oryctolagus cuniculus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.1 additional information
-
-
 Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 L-serine + tetrahydrofolate
-
 Oryctolagus cuniculus glycine + 5,10-methylenetetrahydrofolate + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
2.1.2.1 homotetramer 4 * 53000, SDS-PAGE Oryctolagus cuniculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.2.1 additional information
-
 additional information
-
 Oryctolagus cuniculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.1 tetrahydrofolate requirement with L-serine or L-2-methylserine as substrate Oryctolagus cuniculus
2.1.2.1 tetrahydrofolate allosteric regulation Oryctolagus cuniculus