EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.35 | mature tRNA | weak, competitive inhibitor to methyl-deficient tRNA, non-competitive to S-adenosyl-L-methionine | Escherichia coli | |
2.1.1.35 | S-adenosyl-L-homocysteine | non-competitive to tRNA; product inhibition, competitive to S-adenosyl-L-methionine | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.35 | 0.002 | - |
S-adenosyl-L-methionine | - |
Escherichia coli | |
2.1.1.35 | 0.025 | - |
methyl deficient tRNA | - |
Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.35 | Mg2+ | slight activation | Escherichia coli | |
2.1.1.35 | additional information | not activated by NH4+ | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.35 | S-adenosyl-L-methionine + uridine54 in tRNA | Escherichia coli | specific reaction in maturation of tRNA | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | ribothymidine is the most common methylated nucleoside found in tRNA, it occurs in all tRNAs of the organism | ? | |
2.1.1.35 | S-adenosyl-L-methionine + uridine54 in tRNA | Escherichia coli K12MO7 | specific reaction in maturation of tRNA | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | ribothymidine is the most common methylated nucleoside found in tRNA, it occurs in all tRNAs of the organism | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.35 | Escherichia coli | - |
- |
- |
2.1.1.35 | Escherichia coli K12MO7 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.35 | partial | Escherichia coli |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.1.1.35 | S-adenosyl-L-methionine + uracil54 in tRNA = S-adenosyl-L-homocysteine + 5-methyluracil54 in tRNA | kinetic, sequential mechanism | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.1.1.35 | 0.000167 | - |
- |
Escherichia coli |
EC Number | Storage Stability | Organism |
---|---|---|
2.1.1.35 | -85°C, several months, stable | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.35 | additional information | - |
Escherichia coli | additional information | - |
? | |
2.1.1.35 | additional information | - |
Escherichia coli K12MO7 | additional information | - |
? | |
2.1.1.35 | S-adenosyl-L-methionine + uracil54 in methyl deficient tRNA | - |
Escherichia coli | ? | - |
? | |
2.1.1.35 | S-adenosyl-L-methionine + uracil54 in methyl deficient tRNA | - |
Escherichia coli K12MO7 | ? | - |
? | |
2.1.1.35 | S-adenosyl-L-methionine + uridine54 in tRNA | methyl group acceptor: methyl-deficient tRNA from E. coli | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | one ribothymidine per tRNA molecule, always at the 23rd nucleotide from the 3 end | ? | |
2.1.1.35 | S-adenosyl-L-methionine + uridine54 in tRNA | tRNA and S-adenosyl-L-methionine have separate and independent binding sites | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | one ribothymidine per tRNA molecule, always at the 23rd nucleotide from the 3 end | ? | |
2.1.1.35 | S-adenosyl-L-methionine + uridine54 in tRNA | specific reaction in maturation of tRNA | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | ribothymidine is the most common methylated nucleoside found in tRNA, it occurs in all tRNAs of the organism | ? | |
2.1.1.35 | S-adenosyl-L-methionine + uridine54 in tRNA | methyl group acceptor: methyl-deficient tRNA from E. coli | Escherichia coli K12MO7 | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | one ribothymidine per tRNA molecule, always at the 23rd nucleotide from the 3 end | ? | |
2.1.1.35 | S-adenosyl-L-methionine + uridine54 in tRNA | tRNA and S-adenosyl-L-methionine have separate and independent binding sites | Escherichia coli K12MO7 | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | one ribothymidine per tRNA molecule, always at the 23rd nucleotide from the 3 end | ? | |
2.1.1.35 | S-adenosyl-L-methionine + uridine54 in tRNA | specific reaction in maturation of tRNA | Escherichia coli K12MO7 | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | ribothymidine is the most common methylated nucleoside found in tRNA, it occurs in all tRNAs of the organism | ? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.35 | 9 | - |
- |
Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.35 | S-adenosyl-L-methionine | - |
Escherichia coli |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.35 | 0.0024 | 0.004 | S-adenosyl-L-homocysteine | - |
Escherichia coli | |
2.1.1.35 | 0.03 | 0.1 | mature tRNA | - |
Escherichia coli |